Abstract

Clinical translation of coherent anti-Stokes Raman scattering microscopy is of great interest because of the advantages of noninvasive label-free imaging, high sensitivity, and chemical specificity. For this to happen, we have identified and review the technical barriers that must be overcome. Prior investigations have developed advanced techniques (features), each of which can be used to effectively overcome one particular technical barrier. However, the implementation of one or a small number of these advanced features in previous attempts for clinical translation has often introduced more tradeoffs than benefits. In this review, we outline a strategy that would integrate multiple advanced features to overcome all the technical barriers simultaneously, effectively reduce tradeoffs, and synergistically optimize CARS microscopy for clinical translation. The operation of the envisioned system incorporates coherent Raman micro-spectroscopy for identifying vibrational biomolecular markers of disease and single-frequency (or hyperspectral) Raman imaging of these specific biomarkers for real-time in vivo diagnostics and monitoring.

Original languageEnglish (US)
Pages (from-to)9-22
Number of pages14
JournalJournal of Biophotonics
Volume7
Issue number1-2
DOIs
StatePublished - Jan 1 2014

Fingerprint

Raman Spectrum Analysis
Raman scattering
Microscopy
Microscopic examination
tradeoffs
Raman spectra
microscopy
Imaging techniques
Biomarkers
Labels
biomarkers
Spectroscopy
Sensitivity and Specificity
markers
Monitoring
sensitivity
spectroscopy

Keywords

  • Clinical optical imaging
  • Coherent anti-Stokes Raman scattering microscopy
  • Multiphoton imaging
  • Stimulated Raman scattering microscopy

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Coherent anti-Stokes Raman scattering microscopy : Overcoming technical barriers for clinical translation. / Tu, Haohua; Boppart, Stephen A.

In: Journal of Biophotonics, Vol. 7, No. 1-2, 01.01.2014, p. 9-22.

Research output: Contribution to journalReview article

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