Coccidia-induced mucogenesis promotes the onset of necrotic enteritis by supporting Clostridium perfringens growth

C. T. Collier, C. L. Hofacre, A. M. Payne, D. B. Anderson, P. Kaiser, R. I. Mackie, H. R. Gaskins

Research output: Contribution to journalArticle

Abstract

This study tested the hypothesis that a host mucogenic response to an intestinal coccidial infection promotes the onset of necrotic enteritis (NE). A chick NE model was used in which birds were inoculated with Eimeria acervulina and E. maxima and subsequently with Clostridium perfringens (EAM/CP). A second group of EAM/CP-infected birds was treated with the ionophore narasin (NAR/EAM/CP). These groups were compared to birds that were either non-infected (NIF), or infected only with E. acervulina and E. maxima (EAM), or C. perfringens (CP). The impact of intestinal coccidial infection and anti-coccidial treatment on host immune responses and microbial community structure were evaluated with histochemical-, cultivation- and molecular-based techniques. Barrier function was compromised in EAM/CP-infected birds as indicated by elevated CFUs for anaerobic bacteria and C. perfringens in the spleen when compared to NIF controls at day 20, with a subsequent increase in intestinal NE lesions and mortality at day 22. These results correlate positively with a host inflammatory response as evidenced by increased ileal interleukin (IL)-4, IL-10 and IFN-γ RNA expression. Concurrent increases in chicken intestinal mucin RNA expression, and goblet cell number and theca size indicate that EAM/CP induced an intestinal mucogenic response. Correspondingly, the growth of mucolytic bacteria and C. perfringens as well as α toxin production was greatest in EAM/CP-infected birds. The ionophore narasin, which directly eliminates coccidia, reduced goblet cell theca size, IL-10 and IFN-γ expression, the growth of mucolytic bacteria including C. perfringens, coccidial and NE lesions and mortality in birds that were co-infected with coccidia and C. perfringens. Collectively the data support the hypothesis that coccidial infection induces a host mucogenic response providing a growth advantage to C. perfringens, the causative agent of NE.

Original languageEnglish (US)
Pages (from-to)104-115
Number of pages12
JournalVeterinary Immunology and Immunopathology
Volume122
Issue number1-2
DOIs
StatePublished - Mar 15 2008

Keywords

  • Clostridium perfringens
  • Coccidia
  • Mucus
  • Necrotic enteritis

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Fingerprint Dive into the research topics of 'Coccidia-induced mucogenesis promotes the onset of necrotic enteritis by supporting Clostridium perfringens growth'. Together they form a unique fingerprint.

  • Cite this