Coated substrates employing oriented layers of mutant heme proteins and methods of making and using same

Jill M Olinger; Mark A McLean; Paul W Bohn; Patrick Stayton; Stephen G Sligar

Coated substrates employing oriented layers of mutant heme proteins and methods of making and using same

Jill M Olinger (Inventor), Mark A McLean (Inventor), Paul W Bohn (Inventor), Patrick Stayton (Inventor), Stephen G Sligar (Inventor)

Research output: Patent

Abstract

The present invention is directed to coated substrates having a coating of biological macromolecules, preferably proteins, which are capable of being immobilized on a substrate surface and have a marker. These proteins usually are mutant proteins obtained by mutagenesis of the gene encoding a random positioning protein. When a mutant protein molecule is immobilized on the substrate, the marker of the mutant protein molecule is in a select spatial relationship with both the substrate and the markers of adjacent protein molecules. A substrate coated with an oriented layer of the mutant proteins exhibits improved or different properties when compared to a substrate having a randomly positioned layer of proteins thereon.

Original language	English (US)
U.S. patent number	5466589
Filing date	9/11/92
State	Published - Nov 14 1995

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title = "Coated substrates employing oriented layers of mutant heme proteins and methods of making and using same",

abstract = "The present invention is directed to coated substrates having a coating of biological macromolecules, preferably proteins, which are capable of being immobilized on a substrate surface and have a marker. These proteins usually are mutant proteins obtained by mutagenesis of the gene encoding a random positioning protein. When a mutant protein molecule is immobilized on the substrate, the marker of the mutant protein molecule is in a select spatial relationship with both the substrate and the markers of adjacent protein molecules. A substrate coated with an oriented layer of the mutant proteins exhibits improved or different properties when compared to a substrate having a randomly positioned layer of proteins thereon.",

author = "Olinger, {Jill M} and McLean, {Mark A} and Bohn, {Paul W} and Patrick Stayton and Sligar, {Stephen G}",

note = "The invention described in this application was made in the course of work partially funded by the United States Department of Agriculture, Agriculture Research Service, under Grant No. 59 32U4-7-119. The United States Government may have certain rights in this invention.; 5466589",

year = "1995",

month = nov,

day = "14",

language = "English (US)",

type = "Patent",

}

TY - PAT

T1 - Coated substrates employing oriented layers of mutant heme proteins and methods of making and using same

AU - Olinger, Jill M

AU - McLean, Mark A

AU - Bohn, Paul W

AU - Stayton, Patrick

AU - Sligar, Stephen G

N1 - The invention described in this application was made in the course of work partially funded by the United States Department of Agriculture, Agriculture Research Service, under Grant No. 59 32U4-7-119. The United States Government may have certain rights in this invention.

PY - 1995/11/14

Y1 - 1995/11/14

N2 - The present invention is directed to coated substrates having a coating of biological macromolecules, preferably proteins, which are capable of being immobilized on a substrate surface and have a marker. These proteins usually are mutant proteins obtained by mutagenesis of the gene encoding a random positioning protein. When a mutant protein molecule is immobilized on the substrate, the marker of the mutant protein molecule is in a select spatial relationship with both the substrate and the markers of adjacent protein molecules. A substrate coated with an oriented layer of the mutant proteins exhibits improved or different properties when compared to a substrate having a randomly positioned layer of proteins thereon.

AB - The present invention is directed to coated substrates having a coating of biological macromolecules, preferably proteins, which are capable of being immobilized on a substrate surface and have a marker. These proteins usually are mutant proteins obtained by mutagenesis of the gene encoding a random positioning protein. When a mutant protein molecule is immobilized on the substrate, the marker of the mutant protein molecule is in a select spatial relationship with both the substrate and the markers of adjacent protein molecules. A substrate coated with an oriented layer of the mutant proteins exhibits improved or different properties when compared to a substrate having a randomly positioned layer of proteins thereon.

M3 - Patent

M1 - 5466589

Y2 - 1992/09/11

ER -

Coated substrates employing oriented layers of mutant heme proteins and methods of making and using same

Abstract

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