Abstract
Illinois (IL) and Minnesota (MN) RMV-like strains of barley yellow dwarf virus (BYDV) were identified from maize displaying red leaf symptoms by enzyme- linked immunosorbent assay (ELISA) using antiserum against a New York strain (BYDV-RMV-NY). Some I Land MN strains, but not the NY strain, could be detected by ELISA w'ith a monoclonal antibody raised against BYDV-RPV- NY. The region of the viral genome representing the coat protein gene was amplified by polymerase chain reaction, cloned and sequenced. The nucleotide sequences of the BYDV-RMV-IL and BYDV-RMV-MN coat protein genes differed at just five nucleotide positions while the BYDV-RMV-IL and BYDV- RMV-NY differed at 101 of the 591 positions. The predicted amino acid sequences of the coat proteins of RMV-like strains from IL, MN, and NY shared approximately 60% identity with those of the coat proteins of beet western yellows virus, BYDV-RPV-NY, and potato leafroll virus.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2-5 |
| Number of pages | 4 |
| Journal | Intervirology |
| Volume | 37 |
| Issue number | 1 |
| DOIs | |
| State | Published - 1994 |
Keywords
- Homology
- Maize
- Monoclonal antibodies
- Polymerase chain reaction
ASJC Scopus subject areas
- Virology
- Infectious Diseases