Coat protein sequences of RMV-like strains of barley yellow dwarf virus separate them from other luteoviruses

Leslie L. Domier, Cleora J. D’Arcy, Ludmila I. Lukasheva

Research output: Contribution to journalArticle

Abstract

Illinois (IL) and Minnesota (MN) RMV-like strains of barley yellow dwarf virus (BYDV) were identified from maize displaying red leaf symptoms by enzyme- linked immunosorbent assay (ELISA) using antiserum against a New York strain (BYDV-RMV-NY). Some I Land MN strains, but not the NY strain, could be detected by ELISA w'ith a monoclonal antibody raised against BYDV-RPV- NY. The region of the viral genome representing the coat protein gene was amplified by polymerase chain reaction, cloned and sequenced. The nucleotide sequences of the BYDV-RMV-IL and BYDV-RMV-MN coat protein genes differed at just five nucleotide positions while the BYDV-RMV-IL and BYDV- RMV-NY differed at 101 of the 591 positions. The predicted amino acid sequences of the coat proteins of RMV-like strains from IL, MN, and NY shared approximately 60% identity with those of the coat proteins of beet western yellows virus, BYDV-RPV-NY, and potato leafroll virus.

Original languageEnglish (US)
Pages (from-to)2-5
Number of pages4
JournalIntervirology
Volume37
Issue number1
DOIs
StatePublished - Jan 1 1994

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Luteovirus
Capsid Proteins
Enzyme-Linked Immunosorbent Assay
Viral Genome
Solanum tuberosum
Genes
Zea mays
Immune Sera
Amino Acid Sequence
Nucleotides
Monoclonal Antibodies

Keywords

  • Homology
  • Maize
  • Monoclonal antibodies
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Coat protein sequences of RMV-like strains of barley yellow dwarf virus separate them from other luteoviruses. / Domier, Leslie L.; D’Arcy, Cleora J.; Lukasheva, Ludmila I.

In: Intervirology, Vol. 37, No. 1, 01.01.1994, p. 2-5.

Research output: Contribution to journalArticle

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AU - Lukasheva, Ludmila I.

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AB - Illinois (IL) and Minnesota (MN) RMV-like strains of barley yellow dwarf virus (BYDV) were identified from maize displaying red leaf symptoms by enzyme- linked immunosorbent assay (ELISA) using antiserum against a New York strain (BYDV-RMV-NY). Some I Land MN strains, but not the NY strain, could be detected by ELISA w'ith a monoclonal antibody raised against BYDV-RPV- NY. The region of the viral genome representing the coat protein gene was amplified by polymerase chain reaction, cloned and sequenced. The nucleotide sequences of the BYDV-RMV-IL and BYDV-RMV-MN coat protein genes differed at just five nucleotide positions while the BYDV-RMV-IL and BYDV- RMV-NY differed at 101 of the 591 positions. The predicted amino acid sequences of the coat proteins of RMV-like strains from IL, MN, and NY shared approximately 60% identity with those of the coat proteins of beet western yellows virus, BYDV-RPV-NY, and potato leafroll virus.

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