Co-cladogenesis spanning three phyla: Leafhoppers (Insecta: Hemiptera: Cicadellidae) and their dual bacterial symbionts

Daniela M. Takiya, Phat L. Tran, Christopher H. Dietrich, Nancy A. Moran

Research output: Contribution to journalArticle

Abstract

Endosymbioses are a major form of biological complexity affecting the ecological and evolutionary diversification of many eukaryotic groups. These associations are exemplified by nutritional symbioses of insects for which phylogenetic studies have demonstrated numerous cases of long-term codiversification between a bacterial and a host lineage. Some insects, including most leafhoppers (Insecta: Hemiptera: Cicadellidae), have more than one bacterial symbiont within specialized host cells, raising questions regarding the patterns of codiversification of these multiple partners and the evolutionary persistence of complex symbiotic systems. Previous studies reported the presence of two dominant symbiont types in a member of the leafhopper subfamily Cicadellinae (sharpshooters). In this study, 16S rRNA sequences were obtained and used to examine the occurrence and evolutionary relationships of the two dominant symbiont types across 29 leafhopper species. Candidatus Sulcia muelleri (Bacteroidetes) was detected in all leafhopper species examined, a finding that is consistent with a previous report of its ancient association with the Auchenorrhyncha (a grouping that includes leafhoppers, treehoppers, cicadas, planthoppers, and spittlebugs). Baumannia cicadellinicola (Proteobacteria), previously known from only five sharpshooter species, was found only in the sharpshooter tribes Cicadellini and Proconiini, as well as in the subfamily Phereurhininae. Mitochondrial and nuclear gene sequences were obtained and used to reconstruct host phylogenies. Analyses of host and symbiont data sets support a congruent evolutionary history between sharpshooters, Sulcia and Baumannia and thus provide the first strong evidence for long-term co-inheritance of multiple symbionts during the diversification of a eukaryotic host. Sulcia shows a fivefold lower rate of 16S rDNA sequence divergence than does Baumannia for the same host pairs. The term 'coprimary' symbiont is proposed for such cases.

Original languageEnglish (US)
Pages (from-to)4175-4191
Number of pages17
JournalMolecular ecology
Volume15
Issue number13
DOIs
StatePublished - Oct 26 2006

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Genetic Speciation
Hemiptera
Cicadellidae
symbiont
Insecta
symbionts
Insects
Symbiosis
insect
planthopper
symbiosis
Bacteroidetes
Proteobacteria
Mitochondrial Genes
Sexual Partners
Membracidae
Auchenorrhyncha
Cercopidae
phylogeny
Phylogeny

Keywords

  • Baumannia
  • Cospeciation
  • Mutualism
  • Phylogeny
  • Sulcia

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Co-cladogenesis spanning three phyla : Leafhoppers (Insecta: Hemiptera: Cicadellidae) and their dual bacterial symbionts. / Takiya, Daniela M.; Tran, Phat L.; Dietrich, Christopher H.; Moran, Nancy A.

In: Molecular ecology, Vol. 15, No. 13, 26.10.2006, p. 4175-4191.

Research output: Contribution to journalArticle

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