CNA42 monoclonal antibody identifies FDC as PrPsc accumulating cells in the spleen of scrapie affected sheep

S. Lezmi, A. Bencsik, T. Baron

Research output: Contribution to journalArticlepeer-review

Abstract

Natural scrapie, new variant Creutzfeldt-Jakob disease and murine experimental transmissible spongiform encephalopathies (TSE) are fatal neurodegenerative disorders. The agent responsible for these diseases is closely related to PrPsc, an abnormal isoform of the cellular prion protein. Before reaching the brain, it invades and replicates in lymphoid organs such as spleen, tonsils and lymph nodes. Follicular dendritic cells (FDC) may support the prion replication in lymphoid tissues of sheep as shown in murine models infected with scrapie. In sheep, specific antibodies recognising FDC are lacking. The CNA42 mAb, directed against human FDC was used to identify these cells in sheep spleen. As well as showing that the pre-treatments needed for immunohistochemical detection of PrPsc did not prevent labelling by the CNA42 mAb, accumulation of PrPsc in FDC of spleens of scrapie affected sheep was demonstrated using a double immunolabelling strategy. Thus, the CNA42 antibody represents a suitable tool to identify FDC and investigate their role in natural sheep scrapie.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalVeterinary Immunology and Immunopathology
Volume82
Issue number1-2
DOIs
StatePublished - Sep 28 2001
Externally publishedYes

Keywords

  • CNA42 mAb
  • Follicular dendritic cells (FDC)
  • Lymphoid tissue
  • Prion
  • Scrapie
  • Sheep

ASJC Scopus subject areas

  • Immunology
  • General Veterinary

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