Clustering and jamming in epithelial-mesenchymal co-cultures

Marielena Gamboa Castro, Susan E. Leggett, Ian Y. Wong

Research output: Contribution to journalArticlepeer-review


Collective behaviors emerge from coordinated cell-cell interactions during the morphogenesis of tissues and tumors. For instance, cells may display density-dependent phase transitions from a fluid-like "unjammed" phase to a solid-like "jammed" phase, while different cell types can "self-sort". Here, we comprehensively track single cell dynamics in mixtures of sheet-forming epithelial cells and dispersed mesenchymal cells. We find that proliferating epithelial cells nucleate multicellular clusters that coarsen at a critical density, arresting migration and strengthening spatial velocity correlations. The addition of mesenchymal cells can slow cluster formation and coarsening, resulting in more dispersed individual cells with weak spatial velocity correlations. These behaviors have analogies with a jamming-unjamming transition, where the control parameters are cell density and mesenchymal fraction. This complex interplay of proliferation, clustering and correlated migration may have physical implications for understanding epithelial-mesenchymal interactions in development and disease.

Original languageEnglish (US)
Pages (from-to)8327-8337
Number of pages11
JournalSoft Matter
Issue number40
StatePublished - 2016
Externally publishedYes

ASJC Scopus subject areas

  • General Chemistry
  • Condensed Matter Physics


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