Clostridiolysin S, a post-translationally modified biotoxin from Clostridium botulinum

David J. Gonzalez, Shaun W. Lee, Mary E. Hensler, Andrew L. Markley, Samira Dahesh, Douglas A. Mitchell, Nuno Bandeira, Victor Nizet, Jack E. Dixon, Pieter C. Dorresteina

Research output: Contribution to journalArticle

Abstract

Through elaboration of its botulinum toxins, Clostridium botulinum produces clinical syndromes of infant botulism, wound botulism, and other invasive infections. Using comparative genomic analysis, an orphan nine-gene cluster was identified in C. botulinum and the related foodborne pathogen Clostridium sporogenes that resembled the biosynthetic machinery for streptolysin S, a key virulence factor from group A Streptococcus responsible for its hallmark β-hemolytic phenotype. Genetic complementation, in vitro reconstitution, mass spectral analysis, and plasmid intergrational mutagenesis demonstrate that the streptolysin S-like gene cluster from Clostridium sp. is responsible for the biogenesis of a novel post-translationally modified hemolytic toxin, clostridiolysin S.

Original languageEnglish (US)
Pages (from-to)28220-28228
Number of pages9
JournalJournal of Biological Chemistry
Volume285
Issue number36
DOIs
StatePublished - Sep 3 2010

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Gonzalez, D. J., Lee, S. W., Hensler, M. E., Markley, A. L., Dahesh, S., Mitchell, D. A., Bandeira, N., Nizet, V., Dixon, J. E., & Dorresteina, P. C. (2010). Clostridiolysin S, a post-translationally modified biotoxin from Clostridium botulinum. Journal of Biological Chemistry, 285(36), 28220-28228. https://doi.org/10.1074/jbc.M110.118554