TY - JOUR
T1 - Clostridiolysin S, a post-translationally modified biotoxin from Clostridium botulinum
AU - Gonzalez, David J.
AU - Lee, Shaun W.
AU - Hensler, Mary E.
AU - Markley, Andrew L.
AU - Dahesh, Samira
AU - Mitchell, Douglas A.
AU - Bandeira, Nuno
AU - Nizet, Victor
AU - Dixon, Jack E.
AU - Dorresteina, Pieter C.
PY - 2010/9/3
Y1 - 2010/9/3
N2 - Through elaboration of its botulinum toxins, Clostridium botulinum produces clinical syndromes of infant botulism, wound botulism, and other invasive infections. Using comparative genomic analysis, an orphan nine-gene cluster was identified in C. botulinum and the related foodborne pathogen Clostridium sporogenes that resembled the biosynthetic machinery for streptolysin S, a key virulence factor from group A Streptococcus responsible for its hallmark β-hemolytic phenotype. Genetic complementation, in vitro reconstitution, mass spectral analysis, and plasmid intergrational mutagenesis demonstrate that the streptolysin S-like gene cluster from Clostridium sp. is responsible for the biogenesis of a novel post-translationally modified hemolytic toxin, clostridiolysin S.
AB - Through elaboration of its botulinum toxins, Clostridium botulinum produces clinical syndromes of infant botulism, wound botulism, and other invasive infections. Using comparative genomic analysis, an orphan nine-gene cluster was identified in C. botulinum and the related foodborne pathogen Clostridium sporogenes that resembled the biosynthetic machinery for streptolysin S, a key virulence factor from group A Streptococcus responsible for its hallmark β-hemolytic phenotype. Genetic complementation, in vitro reconstitution, mass spectral analysis, and plasmid intergrational mutagenesis demonstrate that the streptolysin S-like gene cluster from Clostridium sp. is responsible for the biogenesis of a novel post-translationally modified hemolytic toxin, clostridiolysin S.
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U2 - 10.1074/jbc.M110.118554
DO - 10.1074/jbc.M110.118554
M3 - Article
C2 - 20581111
AN - SCOPUS:77956249747
VL - 285
SP - 28220
EP - 28228
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 36
ER -