Closely related families of genes code for the α and α' subunits of the soybean 7S storage protein complex

Mary A Schuler, Eric S. Schmitt, Roger N. Beachy

Research output: Contribution to journalArticle

Abstract

Nineteen cloned cDNAs encoding the α and α' -subunits of the 7S seed storage protein in the soybean, Glycine max, have been isolated from a recombinant cDNA library constructed with mRNA from maturing seeds. In addition, a gene encoding an α' -subunit has been isolated from a recombinant Charon 4A phage library containing genomic Glycine max DNA. The cloned DNAs have been divided, on the basis of their endonuclease sites, into two main classes of sequences which differ in approximately 6% of their nucelotides. Whereas the proteins encoded within each DNA class are nearly identical, the proteins encoded by the two different classes of soybean DNAs are distinct and correspond to α and α' -subunits. Thus, the α and α' ' subunits are coded for by two closely related multigene families. The amino acid differences in the portions of the α and α' -subunits presented in this paper occur primarily near the carboxyl-terminus. The 3' noncoding nucleotides of the cloned α and α' -subunit DNAs are more highly conserved than are the coding nucleotides. This conservation suggests that the 3' untranslated sequences of the α and α' -subunit mRNAs are functional in the expression of the α and α' -subunit proteins or in the stabilization of the 7S subunit mRNAs.

Original languageEnglish (US)
Pages (from-to)8225-8244
Number of pages20
JournalNucleic acids research
Volume10
Issue number24
DOIs
StatePublished - Dec 20 1982
Externally publishedYes

Fingerprint

Storage Protein
Soybean
Soybeans
Messenger RNA
Glycine Max
DNA
Genes
CDNA
Gene
Proteins
Protein
Encoding
Seed Storage
Amino acids
Nucleotides
Glycine
Seed
Pluto
Seed Storage Proteins
Genomics

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Closely related families of genes code for the α and α' subunits of the soybean 7S storage protein complex. / Schuler, Mary A; Schmitt, Eric S.; Beachy, Roger N.

In: Nucleic acids research, Vol. 10, No. 24, 20.12.1982, p. 8225-8244.

Research output: Contribution to journalArticle

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abstract = "Nineteen cloned cDNAs encoding the α and α' -subunits of the 7S seed storage protein in the soybean, Glycine max, have been isolated from a recombinant cDNA library constructed with mRNA from maturing seeds. In addition, a gene encoding an α' -subunit has been isolated from a recombinant Charon 4A phage library containing genomic Glycine max DNA. The cloned DNAs have been divided, on the basis of their endonuclease sites, into two main classes of sequences which differ in approximately 6{\%} of their nucelotides. Whereas the proteins encoded within each DNA class are nearly identical, the proteins encoded by the two different classes of soybean DNAs are distinct and correspond to α and α' -subunits. Thus, the α and α' ' subunits are coded for by two closely related multigene families. The amino acid differences in the portions of the α and α' -subunits presented in this paper occur primarily near the carboxyl-terminus. The 3' noncoding nucleotides of the cloned α and α' -subunit DNAs are more highly conserved than are the coding nucleotides. This conservation suggests that the 3' untranslated sequences of the α and α' -subunit mRNAs are functional in the expression of the α and α' -subunit proteins or in the stabilization of the 7S subunit mRNAs.",
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