Cloning, expression and purification of feline proinsulin

M. Hoenig, Z. F. Caffall, R. A. McGraw, D. C. Ferguson

Research output: Contribution to journalArticlepeer-review

Abstract

Feline proinsulin was cloned and expressed using a bacterial expression system. It was then purified from inclusion bodies using size exclusion chromatography and further processed including reduction of the protein. Following refolding, proinsulin was purified by reversed-phase high-performance liquid chromatography (RP-HPLC). RP-HPLC and mass spectrometric analysis indicated that the proinsulin contained the correct disulfide bridging pattern. This proinsulin can be used for therapeutic and diagnostic purposes.

Original languageEnglish (US)
Pages (from-to)28-37
Number of pages10
JournalDomestic Animal Endocrinology
Volume30
Issue number1
DOIs
StatePublished - Jan 1 2006
Externally publishedYes

Keywords

  • Assay
  • Beta cell
  • Cats
  • Diabetes
  • Insulin

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

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