Cloning, expression and purification of feline proinsulin

M. Hoenig; Z. F. Caffall; R. A. McGraw; D. C. Ferguson

doi:10.1016/j.domaniend.2005.05.005

Cloning, expression and purification of feline proinsulin

M. Hoenig, Z. F. Caffall, R. A. McGraw, D. C. Ferguson

Research output: Contribution to journal › Article › peer-review

Abstract

Feline proinsulin was cloned and expressed using a bacterial expression system. It was then purified from inclusion bodies using size exclusion chromatography and further processed including reduction of the protein. Following refolding, proinsulin was purified by reversed-phase high-performance liquid chromatography (RP-HPLC). RP-HPLC and mass spectrometric analysis indicated that the proinsulin contained the correct disulfide bridging pattern. This proinsulin can be used for therapeutic and diagnostic purposes.

Original language	English (US)
Pages (from-to)	28-37
Number of pages	10
Journal	Domestic Animal Endocrinology
Volume	30
Issue number	1
DOIs	https://doi.org/10.1016/j.domaniend.2005.05.005
State	Published - Jan 2006
Externally published	Yes

Keywords

Assay
Beta cell
Cats
Diabetes
Insulin

ASJC Scopus subject areas

Food Animals
Animal Science and Zoology
Endocrinology

Online availability

10.1016/j.domaniend.2005.05.005

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keywords = "Assay, Beta cell, Cats, Diabetes, Insulin",

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AU - Ferguson, D. C.

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AB - Feline proinsulin was cloned and expressed using a bacterial expression system. It was then purified from inclusion bodies using size exclusion chromatography and further processed including reduction of the protein. Following refolding, proinsulin was purified by reversed-phase high-performance liquid chromatography (RP-HPLC). RP-HPLC and mass spectrometric analysis indicated that the proinsulin contained the correct disulfide bridging pattern. This proinsulin can be used for therapeutic and diagnostic purposes.

KW - Assay

KW - Beta cell

KW - Cats

KW - Diabetes

KW - Insulin

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IS - 1

ER -

Cloning, expression and purification of feline proinsulin

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

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