Cloning, expression and processing of the CP2 neuropeptide precursor of Aplysia

F. S. Vilim; V. Alexeeva; L. L. Moroz; L. Li; T. P. Moroz; J. V. Sweedler; K. R. Weiss

doi:10.1016/S0196-9781(01)00561-7

Cloning, expression and processing of the CP2 neuropeptide precursor of Aplysia

F. S. Vilim
, V. Alexeeva
, L. L. Moroz
, L. Li
, T. P. Moroz
, J. V. Sweedler
, K. R. Weiss

Research output: Contribution to journal › Article › peer-review

Abstract

The cDNA sequence encoding the CP2 neuropeptide precursor is identified and encodes a single copy of the neuropeptide that is flanked by appropriate processing sites. The distribution of the CP2 precursor mRNA is described and matches the CP2-like immunoreactivity described previously. Single cell RT-PCR independently confirms the presence of CP2 precursor mRNA in selected neurons. MALDI-TOF MS is used to identify additional peptides derived from the CP2 precursor in neuronal somata and nerves, suggesting that the CP2 precursor may give rise to additional bioactive neuropeptides.

Original language	English (US)
Pages (from-to)	2027-2038
Number of pages	12
Journal	Peptides
Volume	22
Issue number	12
DOIs	https://doi.org/10.1016/S0196-9781(01)00561-7
State	Published - Dec 2001

Keywords

Aplysia californica
Cerebral peptide 2
In situ hybridization
MALDITOF MS
Neuropeptide
Peptide processing
Single cell RT-PCR
cDNA cloning

ASJC Scopus subject areas

Biochemistry
Physiology
Endocrinology
Cellular and Molecular Neuroscience

Online availability

10.1016/S0196-9781(01)00561-7

Library availability

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@article{4d11d74efc0b40c9bcbdac2791286686,

title = "Cloning, expression and processing of the CP2 neuropeptide precursor of Aplysia",

abstract = "The cDNA sequence encoding the CP2 neuropeptide precursor is identified and encodes a single copy of the neuropeptide that is flanked by appropriate processing sites. The distribution of the CP2 precursor mRNA is described and matches the CP2-like immunoreactivity described previously. Single cell RT-PCR independently confirms the presence of CP2 precursor mRNA in selected neurons. MALDI-TOF MS is used to identify additional peptides derived from the CP2 precursor in neuronal somata and nerves, suggesting that the CP2 precursor may give rise to additional bioactive neuropeptides.",

keywords = "Aplysia californica, Cerebral peptide 2, In situ hybridization, MALDITOF MS, Neuropeptide, Peptide processing, Single cell RT-PCR, cDNA cloning",

author = "Vilim, \{F. S.\} and V. Alexeeva and Moroz, \{L. L.\} and L. Li and Moroz, \{T. P.\} and Sweedler, \{J. V.\} and Weiss, \{K. R.\}",

note = "This work was supported by the National Institutes of Health Grants DA13330, NS31609, NS 39103, MH50235, MH60261, K05MH01427, and NSF CHE 98-77071. The authors gratefully acknowledge the generous gift of Aplysia cDNA library from Dr. Gregg Nagle. Aplysia californica were partially provided by the National Resource for Aplysia at the University of Miami under NIH National Center for Research Resources grant RR10294.",

year = "2001",

month = dec,

doi = "10.1016/S0196-9781(01)00561-7",

language = "English (US)",

volume = "22",

pages = "2027--2038",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "12",

}

TY - JOUR

T1 - Cloning, expression and processing of the CP2 neuropeptide precursor of Aplysia

AU - Vilim, F. S.

AU - Alexeeva, V.

AU - Moroz, L. L.

AU - Li, L.

AU - Moroz, T. P.

AU - Sweedler, J. V.

AU - Weiss, K. R.

N1 - This work was supported by the National Institutes of Health Grants DA13330, NS31609, NS 39103, MH50235, MH60261, K05MH01427, and NSF CHE 98-77071. The authors gratefully acknowledge the generous gift of Aplysia cDNA library from Dr. Gregg Nagle. Aplysia californica were partially provided by the National Resource for Aplysia at the University of Miami under NIH National Center for Research Resources grant RR10294.

PY - 2001/12

Y1 - 2001/12

N2 - The cDNA sequence encoding the CP2 neuropeptide precursor is identified and encodes a single copy of the neuropeptide that is flanked by appropriate processing sites. The distribution of the CP2 precursor mRNA is described and matches the CP2-like immunoreactivity described previously. Single cell RT-PCR independently confirms the presence of CP2 precursor mRNA in selected neurons. MALDI-TOF MS is used to identify additional peptides derived from the CP2 precursor in neuronal somata and nerves, suggesting that the CP2 precursor may give rise to additional bioactive neuropeptides.

AB - The cDNA sequence encoding the CP2 neuropeptide precursor is identified and encodes a single copy of the neuropeptide that is flanked by appropriate processing sites. The distribution of the CP2 precursor mRNA is described and matches the CP2-like immunoreactivity described previously. Single cell RT-PCR independently confirms the presence of CP2 precursor mRNA in selected neurons. MALDI-TOF MS is used to identify additional peptides derived from the CP2 precursor in neuronal somata and nerves, suggesting that the CP2 precursor may give rise to additional bioactive neuropeptides.

KW - Aplysia californica

KW - Cerebral peptide 2

KW - In situ hybridization

KW - MALDITOF MS

KW - Neuropeptide

KW - Peptide processing

KW - Single cell RT-PCR

KW - cDNA cloning

UR - https://www.scopus.com/pages/publications/0035754885

UR - https://www.scopus.com/pages/publications/0035754885#tab=citedBy

U2 - 10.1016/S0196-9781(01)00561-7

DO - 10.1016/S0196-9781(01)00561-7

M3 - Article

C2 - 11786187

AN - SCOPUS:0035754885

SN - 0196-9781

VL - 22

SP - 2027

EP - 2038

JO - Peptides

JF - Peptides

IS - 12

ER -

Cloning, expression and processing of the CP2 neuropeptide precursor of Aplysia

Abstract

Keywords

ASJC Scopus subject areas

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