Cloning, expression and processing of the CP2 neuropeptide precursor of Aplysia

F. S. Vilim, V. Alexeeva, L. L. Moroz, L. Li, T. P. Moroz, J. V. Sweedler, K. R. Weiss

Research output: Contribution to journalArticlepeer-review


The cDNA sequence encoding the CP2 neuropeptide precursor is identified and encodes a single copy of the neuropeptide that is flanked by appropriate processing sites. The distribution of the CP2 precursor mRNA is described and matches the CP2-like immunoreactivity described previously. Single cell RT-PCR independently confirms the presence of CP2 precursor mRNA in selected neurons. MALDI-TOF MS is used to identify additional peptides derived from the CP2 precursor in neuronal somata and nerves, suggesting that the CP2 precursor may give rise to additional bioactive neuropeptides.

Original languageEnglish (US)
Pages (from-to)2027-2038
Number of pages12
Issue number12
StatePublished - Dec 2001


  • Aplysia californica
  • Cerebral peptide 2
  • In situ hybridization
  • Neuropeptide
  • Peptide processing
  • Single cell RT-PCR
  • cDNA cloning

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Endocrinology
  • Cellular and Molecular Neuroscience


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