Cloning, characterization and sequence comparison of the gene coding for IMP dehydrogenase from Pyrococcus furiosus

Frank R. Collart, Jerzy Osipiuk, Jonathan Trent, Gary J. Olsen, Eliezer Huberman

Research output: Contribution to journalArticle

Abstract

We have cloned and characterized the gene encoding inosine monophosphate dehydrogenase (IMPDH) from Pyrococcus furiosus (Pf), a hyperthermophillic archeon. Sequence analysis of the Pf gene indicated an open reading frame specifying a protein of 485 amino acids (aa) with a calculated M(r) of 52900. Canonical Archaea promoter elements, Box A and Box B, are located -49 and -17 nucleotides (nt), respectively, upstream of the putative start codon. The sequence of the putative active-site region conforms to the IMPDH signature motif and contains a putative active-site cysteine. Phylogenetic relationships derived by using all available IMPDH sequences are consistent with trees developed for other molecules; they do not precisely resolve the history of Pf IMPDH but indicate a close similarity to bacterial IMPDH proteins. The phylogenetic analysis indicates that a gene duplication occurred prior to the division between rodents and humans, accounting for the Type I and II isoforms identified in mice and humans.

Original languageEnglish (US)
Pages (from-to)209-216
Number of pages8
JournalGene
Volume174
Issue number2
DOIs
StatePublished - Jan 1 1996

Keywords

  • Archae
  • DNA sequence
  • Gene isolation
  • Phylogeny
  • Protein homology

ASJC Scopus subject areas

  • Genetics

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