Cloning, characterization and sequence comparison of the gene coding for IMP dehydrogenase from Pyrococcus furiosus

Frank R. Collart, Jerzy Osipiuk, Jonathan Trent, Gary J. Olsen, Eliezer Huberman

Research output: Contribution to journalArticle

Abstract

We have cloned and characterized the gene encoding inosine monophosphate dehydrogenase (IMPDH) from Pyrococcus furiosus (Pf), a hyperthermophillic archeon. Sequence analysis of the Pf gene indicated an open reading frame specifying a protein of 485 amino acids (aa) with a calculated M(r) of 52900. Canonical Archaea promoter elements, Box A and Box B, are located -49 and -17 nucleotides (nt), respectively, upstream of the putative start codon. The sequence of the putative active-site region conforms to the IMPDH signature motif and contains a putative active-site cysteine. Phylogenetic relationships derived by using all available IMPDH sequences are consistent with trees developed for other molecules; they do not precisely resolve the history of Pf IMPDH but indicate a close similarity to bacterial IMPDH proteins. The phylogenetic analysis indicates that a gene duplication occurred prior to the division between rodents and humans, accounting for the Type I and II isoforms identified in mice and humans.

Original languageEnglish (US)
Pages (from-to)209-216
Number of pages8
JournalGene
Volume174
Issue number2
DOIs
StatePublished - Jan 1 1996

Fingerprint

IMP Dehydrogenase
Pyrococcus furiosus
Inosine Monophosphate
Organism Cloning
Oxidoreductases
Genes
Archaea
Catalytic Domain
Gene Duplication
Initiator Codon
Open Reading Frames
Cysteine
Sequence Analysis
Rodentia
Protein Isoforms
Proteins
Nucleotides
History
Amino Acids

Keywords

  • Archae
  • DNA sequence
  • Gene isolation
  • Phylogeny
  • Protein homology

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning, characterization and sequence comparison of the gene coding for IMP dehydrogenase from Pyrococcus furiosus. / Collart, Frank R.; Osipiuk, Jerzy; Trent, Jonathan; Olsen, Gary J.; Huberman, Eliezer.

In: Gene, Vol. 174, No. 2, 01.01.1996, p. 209-216.

Research output: Contribution to journalArticle

Collart, Frank R. ; Osipiuk, Jerzy ; Trent, Jonathan ; Olsen, Gary J. ; Huberman, Eliezer. / Cloning, characterization and sequence comparison of the gene coding for IMP dehydrogenase from Pyrococcus furiosus. In: Gene. 1996 ; Vol. 174, No. 2. pp. 209-216.
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