Abstract
Messenger RNA of the calcium-sensing receptor from feline parathyroid gland (fCaSR) was reversed transcribed to cDNA, amplified by polymerase chain reaction (PCR) and cloned into E. coli. Sequences obtained from cloned E. coli were used for genetic characterization of the fCaSR mRNA and for exonic PCR primer design. Multiple fCaSR exons sequence alignments obtained from PCR amplification of genomic DNA of 5 healthy domestic shorthair cats indicated the presence of 3 synonymous missense single-nucleotide polymorphisms (SNP) and 1 nonsynonymous missense SNP, which changed an amino acid from arginine to proline. The fCaSR has 96%, 96%, and 94% homology to the canine, human, and bovine amino acid sequences, respectively.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 57-61 |
| Number of pages | 5 |
| Journal | Domestic Animal Endocrinology |
| Volume | 38 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2010 |
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Keywords
- Calcium homeostasis
- Calcium-sensing receptor
- Feline
- Parathyroid gland
ASJC Scopus subject areas
- Food Animals
- Animal Science and Zoology
- Endocrinology
Cite this
Cloning and sequencing of the calcium-sensing receptor from the feline parathyroid gland. / Gal, A.; Ridge, T. K.; Graves, T. K.
In: Domestic Animal Endocrinology, Vol. 38, No. 1, 01.01.2010, p. 57-61.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and sequencing of the calcium-sensing receptor from the feline parathyroid gland
AU - Gal, A.
AU - Ridge, T. K.
AU - Graves, T. K.
PY - 2010/1/1
Y1 - 2010/1/1
N2 - Messenger RNA of the calcium-sensing receptor from feline parathyroid gland (fCaSR) was reversed transcribed to cDNA, amplified by polymerase chain reaction (PCR) and cloned into E. coli. Sequences obtained from cloned E. coli were used for genetic characterization of the fCaSR mRNA and for exonic PCR primer design. Multiple fCaSR exons sequence alignments obtained from PCR amplification of genomic DNA of 5 healthy domestic shorthair cats indicated the presence of 3 synonymous missense single-nucleotide polymorphisms (SNP) and 1 nonsynonymous missense SNP, which changed an amino acid from arginine to proline. The fCaSR has 96%, 96%, and 94% homology to the canine, human, and bovine amino acid sequences, respectively.
AB - Messenger RNA of the calcium-sensing receptor from feline parathyroid gland (fCaSR) was reversed transcribed to cDNA, amplified by polymerase chain reaction (PCR) and cloned into E. coli. Sequences obtained from cloned E. coli were used for genetic characterization of the fCaSR mRNA and for exonic PCR primer design. Multiple fCaSR exons sequence alignments obtained from PCR amplification of genomic DNA of 5 healthy domestic shorthair cats indicated the presence of 3 synonymous missense single-nucleotide polymorphisms (SNP) and 1 nonsynonymous missense SNP, which changed an amino acid from arginine to proline. The fCaSR has 96%, 96%, and 94% homology to the canine, human, and bovine amino acid sequences, respectively.
KW - Calcium homeostasis
KW - Calcium-sensing receptor
KW - Feline
KW - Parathyroid gland
UR - http://www.scopus.com/inward/record.url?scp=70449640191&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70449640191&partnerID=8YFLogxK
U2 - 10.1016/j.domaniend.2009.07.004
DO - 10.1016/j.domaniend.2009.07.004
M3 - Article
C2 - 19700256
AN - SCOPUS:70449640191
VL - 38
SP - 57
EP - 61
JO - Domestic Animal Endocrinology
JF - Domestic Animal Endocrinology
SN - 0739-7240
IS - 1
ER -