Cloning and sequencing of the calcium-sensing receptor from the feline parathyroid gland

A. Gal; T. K. Ridge; T. K. Graves

doi:10.1016/j.domaniend.2009.07.004

Cloning and sequencing of the calcium-sensing receptor from the feline parathyroid gland

A. Gal, T. K. Ridge, T. K. Graves

Research output: Contribution to journal › Article › peer-review

Abstract

Messenger RNA of the calcium-sensing receptor from feline parathyroid gland (fCaSR) was reversed transcribed to cDNA, amplified by polymerase chain reaction (PCR) and cloned into E. coli. Sequences obtained from cloned E. coli were used for genetic characterization of the fCaSR mRNA and for exonic PCR primer design. Multiple fCaSR exons sequence alignments obtained from PCR amplification of genomic DNA of 5 healthy domestic shorthair cats indicated the presence of 3 synonymous missense single-nucleotide polymorphisms (SNP) and 1 nonsynonymous missense SNP, which changed an amino acid from arginine to proline. The fCaSR has 96%, 96%, and 94% homology to the canine, human, and bovine amino acid sequences, respectively.

Original language	English (US)
Pages (from-to)	57-61
Number of pages	5
Journal	Domestic Animal Endocrinology
Volume	38
Issue number	1
DOIs	https://doi.org/10.1016/j.domaniend.2009.07.004
State	Published - Jan 2010

Keywords

Calcium homeostasis
Calcium-sensing receptor
Feline
Parathyroid gland

ASJC Scopus subject areas

Food Animals
Animal Science and Zoology
Endocrinology

Online availability

10.1016/j.domaniend.2009.07.004

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abstract = "Messenger RNA of the calcium-sensing receptor from feline parathyroid gland (fCaSR) was reversed transcribed to cDNA, amplified by polymerase chain reaction (PCR) and cloned into E. coli. Sequences obtained from cloned E. coli were used for genetic characterization of the fCaSR mRNA and for exonic PCR primer design. Multiple fCaSR exons sequence alignments obtained from PCR amplification of genomic DNA of 5 healthy domestic shorthair cats indicated the presence of 3 synonymous missense single-nucleotide polymorphisms (SNP) and 1 nonsynonymous missense SNP, which changed an amino acid from arginine to proline. The fCaSR has 96%, 96%, and 94% homology to the canine, human, and bovine amino acid sequences, respectively.",

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AU - Ridge, T. K.

AU - Graves, T. K.

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AB - Messenger RNA of the calcium-sensing receptor from feline parathyroid gland (fCaSR) was reversed transcribed to cDNA, amplified by polymerase chain reaction (PCR) and cloned into E. coli. Sequences obtained from cloned E. coli were used for genetic characterization of the fCaSR mRNA and for exonic PCR primer design. Multiple fCaSR exons sequence alignments obtained from PCR amplification of genomic DNA of 5 healthy domestic shorthair cats indicated the presence of 3 synonymous missense single-nucleotide polymorphisms (SNP) and 1 nonsynonymous missense SNP, which changed an amino acid from arginine to proline. The fCaSR has 96%, 96%, and 94% homology to the canine, human, and bovine amino acid sequences, respectively.

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Cloning and sequencing of the calcium-sensing receptor from the feline parathyroid gland

Abstract

Keywords

ASJC Scopus subject areas

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