Cloning and sequencing of the calcium-sensing receptor from the feline parathyroid gland

A. Gal, T. K. Ridge, T. K. Graves

Research output: Contribution to journalArticle

Abstract

Messenger RNA of the calcium-sensing receptor from feline parathyroid gland (fCaSR) was reversed transcribed to cDNA, amplified by polymerase chain reaction (PCR) and cloned into E. coli. Sequences obtained from cloned E. coli were used for genetic characterization of the fCaSR mRNA and for exonic PCR primer design. Multiple fCaSR exons sequence alignments obtained from PCR amplification of genomic DNA of 5 healthy domestic shorthair cats indicated the presence of 3 synonymous missense single-nucleotide polymorphisms (SNP) and 1 nonsynonymous missense SNP, which changed an amino acid from arginine to proline. The fCaSR has 96%, 96%, and 94% homology to the canine, human, and bovine amino acid sequences, respectively.

Original languageEnglish (US)
Pages (from-to)57-61
Number of pages5
JournalDomestic Animal Endocrinology
Volume38
Issue number1
DOIs
StatePublished - Jan 1 2010

Fingerprint

parathyroid glands
Calcium-Sensing Receptors
Parathyroid Glands
Felidae
Organism Cloning
molecular cloning
polymerase chain reaction
cats
calcium
Polymerase Chain Reaction
receptors
single nucleotide polymorphism
Single Nucleotide Polymorphism
Escherichia coli
Messenger RNA
Sequence Alignment
sequence alignment
messenger RNA
Proline
exons

Keywords

  • Calcium homeostasis
  • Calcium-sensing receptor
  • Feline
  • Parathyroid gland

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology
  • Endocrinology

Cite this

Cloning and sequencing of the calcium-sensing receptor from the feline parathyroid gland. / Gal, A.; Ridge, T. K.; Graves, T. K.

In: Domestic Animal Endocrinology, Vol. 38, No. 1, 01.01.2010, p. 57-61.

Research output: Contribution to journalArticle

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