Abstract
Mouse nestin genomic DNA (23.3 kb) was obtained after genomic library screening. Sequence comparison of the cDNA and genomic DNA revealed three introns in this gene. The positions and length of these introns are conserved among rat and human. The effect of the second intron of mouse nestin gene on the expression of luciferase reporter gene was investigated in vitro. Transient transfection of the second intron and its series deletions of mouse nestin gene showed that the second intron of mouse nestin gene could enhance reporter gene expression and the activity was contributed by a fragment about 800 bp long of its 3 ′ end.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 651-652 |
| Number of pages | 2 |
| Journal | Acta Biochimica et Biophysica Sinica |
| Volume | 32 |
| Issue number | 6 |
| State | Published - 2000 |
| Externally published | Yes |
Keywords
- Gene structure
- Mouse
- Nestin gene
- Regulatory element
ASJC Scopus subject areas
- Biophysics
- Biochemistry