Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli

Kelly Magnuson; Won Oh; Timothy J. Larson; John E. Cronan

doi:10.1016/0014-5793(92)80128-4

Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli

Kelly Magnuson, Won Oh, Timothy J. Larson, John E. Cronan

Research output: Contribution to journal › Article › peer-review

Abstract

We report the cloning and nucleotide sequence of the gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli, Malonyl transacylase has been overexpressed 155-fold compared to a wild-type strain, Overexpression of this enzyme alters the fatty acid composition of a wild-type E. coli strain; increased amounts of cis-vaccenate are incorporated into the membrane phospholipids.

Original language	English (US)
Pages (from-to)	262-266
Number of pages	5
Journal	FEBS Letters
Volume	299
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(92)80128-4
State	Published - Mar 1992

Keywords

Acyl transferase
Fatty acid biosynthesis
Malonyl transacylase
Malonyl-CoA

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Online availability

10.1016/0014-5793(92)80128-4

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title = "Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli",

abstract = "We report the cloning and nucleotide sequence of the gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli, Malonyl transacylase has been overexpressed 155-fold compared to a wild-type strain, Overexpression of this enzyme alters the fatty acid composition of a wild-type E. coli strain; increased amounts of cis-vaccenate are incorporated into the membrane phospholipids.",

keywords = "Acyl transferase, Fatty acid biosynthesis, Malonyl transacylase, Malonyl-CoA",

author = "Kelly Magnuson and Won Oh and Larson, {Timothy J.} and Cronan, {John E.}",

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year = "1992",

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doi = "10.1016/0014-5793(92)80128-4",

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pages = "262--266",

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TY - JOUR

T1 - Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli

AU - Magnuson, Kelly

AU - Oh, Won

AU - Larson, Timothy J.

AU - Cronan, John E.

N1 - Funding Information: AckttoH~(e~~e~rettrTsh: is work was supportedb y the National In-stituteso f HealthG rant Al 15650(J .E.C.) and a NationalI nstitutes of HealthB iomedicaRl esearchS upportG rant (T.J.L.). Ali T. van LooaBhattacharyisa g ratefullya cknowledgefdo r skillful1t echnical assistanceW. e thankY . Kohara for phages2 32t hrough2 37o f the miniseta, nd Yuyun Li for thec onstructiona nd sequencinogf some of the M I3 clones.

PY - 1992/3

Y1 - 1992/3

N2 - We report the cloning and nucleotide sequence of the gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli, Malonyl transacylase has been overexpressed 155-fold compared to a wild-type strain, Overexpression of this enzyme alters the fatty acid composition of a wild-type E. coli strain; increased amounts of cis-vaccenate are incorporated into the membrane phospholipids.

AB - We report the cloning and nucleotide sequence of the gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli, Malonyl transacylase has been overexpressed 155-fold compared to a wild-type strain, Overexpression of this enzyme alters the fatty acid composition of a wild-type E. coli strain; increased amounts of cis-vaccenate are incorporated into the membrane phospholipids.

KW - Acyl transferase

KW - Fatty acid biosynthesis

KW - Malonyl transacylase

KW - Malonyl-CoA

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U2 - 10.1016/0014-5793(92)80128-4

DO - 10.1016/0014-5793(92)80128-4

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SN - 0014-5793

VL - 299

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JO - FEBS Letters

JF - FEBS Letters

IS - 3

ER -

Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli

Abstract

Keywords

ASJC Scopus subject areas

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