Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli

Kelly Magnuson, Won Oh, Timothy J. Larson, John E Cronan

Research output: Contribution to journalArticle

Abstract

We report the cloning and nucleotide sequence of the gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli, Malonyl transacylase has been overexpressed 155-fold compared to a wild-type strain, Overexpression of this enzyme alters the fatty acid composition of a wild-type E. coli strain; increased amounts of cis-vaccenate are incorporated into the membrane phospholipids.

Original languageEnglish (US)
Pages (from-to)262-266
Number of pages5
JournalFEBS Letters
Volume299
Issue number3
DOIs
StatePublished - Mar 1992

Keywords

  • Acyl transferase
  • Fatty acid biosynthesis
  • Malonyl transacylase
  • Malonyl-CoA

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Cloning and nucleotide sequence of the fabD gene encoding malonyl coenzyme A-acyl carrier protein transacylase of Escherichia coli'. Together they form a unique fingerprint.

  • Cite this