Abstract
We have cloned and characterized the gene encoding inosine monophosphate dehydrogenase (IMPDH) from Arabidopsis thaliana (At). The transcription unit of the At gene spans approximately 1900 bp and specifies a protein of 503 amino acids with a calculated relative molecular mass (M(r)) of 54,190. The gene is comprised of a minimum of four introns and five exons with all donor and acceptor splice sequences conforming to previously proposed consensus sequences. The deduced IMPDH amino-acid sequence from At shows a remarkable similarity to other eukaryotic IMPDH sequences, with a 48% identity to human Type II enzyme. Allowing for conservative substitutions, the enzyme is 69% similar to human Type II IMPDH. The putative active-site sequence of At IMPDH conforms to the IMP dehydrogenase/guanosine monophosphate reductase motif and contains an essential active-site cysteine residue.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 217-220 |
| Number of pages | 4 |
| Journal | Gene |
| Volume | 174 |
| Issue number | 2 |
| DOIs | |
| State | Published - Jan 1 1996 |
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Keywords
- DNA sequence
- Gene isolation
- Plant
- Protein homology
ASJC Scopus subject areas
- Genetics
Cite this
Cloning and characterization of the gene encoding IMP dehydrogenase from Arabidopsis thaliana. / Collart, Frank R.; Osipiuk, Jerzy; Trent, Jonathan; Olsen, Gary J.; Huberman, Eliezer.
In: Gene, Vol. 174, No. 2, 01.01.1996, p. 217-220.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and characterization of the gene encoding IMP dehydrogenase from Arabidopsis thaliana
AU - Collart, Frank R.
AU - Osipiuk, Jerzy
AU - Trent, Jonathan
AU - Olsen, Gary J.
AU - Huberman, Eliezer
PY - 1996/1/1
Y1 - 1996/1/1
N2 - We have cloned and characterized the gene encoding inosine monophosphate dehydrogenase (IMPDH) from Arabidopsis thaliana (At). The transcription unit of the At gene spans approximately 1900 bp and specifies a protein of 503 amino acids with a calculated relative molecular mass (M(r)) of 54,190. The gene is comprised of a minimum of four introns and five exons with all donor and acceptor splice sequences conforming to previously proposed consensus sequences. The deduced IMPDH amino-acid sequence from At shows a remarkable similarity to other eukaryotic IMPDH sequences, with a 48% identity to human Type II enzyme. Allowing for conservative substitutions, the enzyme is 69% similar to human Type II IMPDH. The putative active-site sequence of At IMPDH conforms to the IMP dehydrogenase/guanosine monophosphate reductase motif and contains an essential active-site cysteine residue.
AB - We have cloned and characterized the gene encoding inosine monophosphate dehydrogenase (IMPDH) from Arabidopsis thaliana (At). The transcription unit of the At gene spans approximately 1900 bp and specifies a protein of 503 amino acids with a calculated relative molecular mass (M(r)) of 54,190. The gene is comprised of a minimum of four introns and five exons with all donor and acceptor splice sequences conforming to previously proposed consensus sequences. The deduced IMPDH amino-acid sequence from At shows a remarkable similarity to other eukaryotic IMPDH sequences, with a 48% identity to human Type II enzyme. Allowing for conservative substitutions, the enzyme is 69% similar to human Type II IMPDH. The putative active-site sequence of At IMPDH conforms to the IMP dehydrogenase/guanosine monophosphate reductase motif and contains an essential active-site cysteine residue.
KW - DNA sequence
KW - Gene isolation
KW - Plant
KW - Protein homology
UR - http://www.scopus.com/inward/record.url?scp=0029845410&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029845410&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(96)00045-5
DO - 10.1016/0378-1119(96)00045-5
M3 - Article
C2 - 8890737
AN - SCOPUS:0029845410
VL - 174
SP - 217
EP - 220
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -