Cloning and characterization of the gene encoding IMP dehydrogenase from Arabidopsis thaliana

Frank R. Collart, Jerzy Osipiuk, Jonathan Trent, Gary J. Olsen, Eliezer Huberman

Research output: Contribution to journalArticle

Abstract

We have cloned and characterized the gene encoding inosine monophosphate dehydrogenase (IMPDH) from Arabidopsis thaliana (At). The transcription unit of the At gene spans approximately 1900 bp and specifies a protein of 503 amino acids with a calculated relative molecular mass (M(r)) of 54,190. The gene is comprised of a minimum of four introns and five exons with all donor and acceptor splice sequences conforming to previously proposed consensus sequences. The deduced IMPDH amino-acid sequence from At shows a remarkable similarity to other eukaryotic IMPDH sequences, with a 48% identity to human Type II enzyme. Allowing for conservative substitutions, the enzyme is 69% similar to human Type II IMPDH. The putative active-site sequence of At IMPDH conforms to the IMP dehydrogenase/guanosine monophosphate reductase motif and contains an essential active-site cysteine residue.

Original languageEnglish (US)
Pages (from-to)217-220
Number of pages4
JournalGene
Volume174
Issue number2
DOIs
StatePublished - Jan 1 1996

Fingerprint

IMP Dehydrogenase
Inosine Monophosphate
Arabidopsis
Organism Cloning
Oxidoreductases
GMP Reductase
Genes
Catalytic Domain
Consensus Sequence
Enzymes
Introns
Cysteine
Amino Acid Sequence
Exons
Amino Acids
Proteins

Keywords

  • DNA sequence
  • Gene isolation
  • Plant
  • Protein homology

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning and characterization of the gene encoding IMP dehydrogenase from Arabidopsis thaliana. / Collart, Frank R.; Osipiuk, Jerzy; Trent, Jonathan; Olsen, Gary J.; Huberman, Eliezer.

In: Gene, Vol. 174, No. 2, 01.01.1996, p. 217-220.

Research output: Contribution to journalArticle

Collart, Frank R. ; Osipiuk, Jerzy ; Trent, Jonathan ; Olsen, Gary J. ; Huberman, Eliezer. / Cloning and characterization of the gene encoding IMP dehydrogenase from Arabidopsis thaliana. In: Gene. 1996 ; Vol. 174, No. 2. pp. 217-220.
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