Cloning and analysing of 5' flanking region of Xenopus organizer gene noggin

Qin Hua Tao, Jing Yang, Wenyan Mei, Xin Geng, Xiao Yan Ding

Research output: Contribution to journalArticlepeer-review

Abstract

Xenopus organizer specific gene Noggin possesses nearly all the characterestic properties of the action of organizer to specify the embryonic body axis. To analyze how the maternal inherited factors control its expression pattern, we cloned the 5' regulatory region of noggin gene. The 1.5 kb upstream sequense could direct reporter gene to express in vivo and data from deletion analysis indicated that a 229 base pair fragmet is essential for activating noggin expression. We further demonstrated that the response elements within this regulatory region were indeed under the control of growth factor activin and Wnt signaling pathway components.

Original languageEnglish (US)
Pages (from-to)209-216
Number of pages8
JournalCell Research
Volume9
Issue number3
DOIs
StatePublished - Sep 1999
Externally publishedYes

Keywords

  • Noggin
  • Organizer specific gene
  • Regulatory region
  • Xenopus

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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