TY - JOUR
T1 - Classical swine fever virus isolates from Cuba form a new subgenotype 1.4
AU - Postel, Alexander
AU - Schmeiser, Stefanie
AU - Perera, Carmen Laura
AU - Rodríguez, Lester Josué Pérez
AU - Frias-Lepoureau, Maria Teresa
AU - Becher, Paul
N1 - Funding Information:
This study was supported by an OIE Twinning project grant “Support to the CENSA Laboratory for CSF for the planned establishment of an OIE Reference Laboratory”.
PY - 2013/1/25
Y1 - 2013/1/25
N2 - Identification and classification of classical swine fever virus (CSFV) on the basis of nucleotide sequencing and phylogenetic analysis have become an important tool to study the epidemiology and to control CSF disease. According to phylogenetic analyses of short sequences from the 5'nontranslated region (150nt) and the E2 (190nt), most CSFV isolates from South and Central America have been assorted to the subgenotypes 1.1 and 1.3, while CSFV isolates from Cuba have been allocated to subgenotype 1.2. Here we demonstrate that determination and comparison of full-length E2 sequences as well as of the sequences encoding for Npro, C, Erns, E1 and E2 (3361nt) do not support segregation of Cuban CSFV isolates to subgenotype 1.2. In fact, our analysis revealed that the Cuban isolates are more divergent from other so far known CSFV subgenotype 1 isolates and form a novel separate subgenotype that is proposed to be designated subgenotype 1.4.
AB - Identification and classification of classical swine fever virus (CSFV) on the basis of nucleotide sequencing and phylogenetic analysis have become an important tool to study the epidemiology and to control CSF disease. According to phylogenetic analyses of short sequences from the 5'nontranslated region (150nt) and the E2 (190nt), most CSFV isolates from South and Central America have been assorted to the subgenotypes 1.1 and 1.3, while CSFV isolates from Cuba have been allocated to subgenotype 1.2. Here we demonstrate that determination and comparison of full-length E2 sequences as well as of the sequences encoding for Npro, C, Erns, E1 and E2 (3361nt) do not support segregation of Cuban CSFV isolates to subgenotype 1.2. In fact, our analysis revealed that the Cuban isolates are more divergent from other so far known CSFV subgenotype 1 isolates and form a novel separate subgenotype that is proposed to be designated subgenotype 1.4.
KW - Classical swine fever virus
KW - Cuba
KW - Phylogenetic analysis
KW - Subgenotype 1.4
UR - http://www.scopus.com/inward/record.url?scp=84870393340&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84870393340&partnerID=8YFLogxK
U2 - 10.1016/j.vetmic.2012.07.045
DO - 10.1016/j.vetmic.2012.07.045
M3 - Article
C2 - 22902191
AN - SCOPUS:84870393340
SN - 0378-1135
VL - 161
SP - 334
EP - 338
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 3-4
ER -