Circulating progenitor cells are positively associated with cognitive function among overweight/obese children

Grace M. Niemiro, Lauren B. Raine, Naiman A. Khan, Russell Emmons, Jonathan Little, Arthur F. Kramer, Charles H. Hillman, Michael De Lisio

Research output: Contribution to journalArticlepeer-review

Abstract

Recent evidence has indicated that overweight/obese children may experience cognitive and immune dysfunction, but the underlying mechanisms responsible for the association between overweight/obesity, immune dysfunction, and cognition have yet to be established. The present study aimed to identify a novel link between obesity-induced immune system dysregulation and cognition in preadolescent children. A total of 27 male children (age: 8–10 years) were recruited and separated by body mass index (BMI) into healthy weight (HW: 5th–84.9th percentile, n = 16) and overweight/obese (OW: ⩾85th percentile, n = 11) groups. Adiposity was assessed using dual energy X-ray absorptiometry (DXA), and aspects of executive function were assessed using the Woodcock-Johnson III Tests of Cognitive Abilities. Monocyte populations (CD14+CD16, CD14+CD16+) with and without expression of chemokine receptor type 2 (CCR2), and circulating progenitor cells (CPCs: CD34+CD45dim), in peripheral blood were quantified by flow cytometry. CPCs were isolated by flow sorting and cultured for 24 h for collection of conditioned media (CM) that was applied to SH-SY5Y neuroblastomas to examine the paracrine effects of CPCs on neurogenesis. OW had significantly higher quantities of both populations of monocytes (CD14+CD16: 57% increase; CD14+CD16+: 95% increase, both p < 0.01), monocytes expressing CCR2 (CD14+CD16CCR2+: 66% increase; CD14+CD16+CCR2+: 168% increase, both p < 0.01), and CPCs (47% increase, p < 0.05) than HW. CPCs were positively correlated with abdominal adiposity in OW, and negatively correlated in HW with a significant difference between correlations (p < 0.05). CPC content was positively correlated with executive processes in OW, and negatively correlated in HW with a significant difference in the strength of the correlations between groups (p < 0.05 for correlation between OW and HW). Finally, CPC-CM from OW trended to increase neuroblast viability in vitro relative to HW (1.79 fold, p = 0.07). These novel findings indicate that increased content of CPCs among OW children may play a role in preventing decrements in cognitive function via paracrine mechanisms.

Original languageEnglish (US)
Pages (from-to)47-52
Number of pages6
JournalBrain, Behavior, and Immunity
Volume57
DOIs
StatePublished - Oct 1 2016

Keywords

  • Executive function
  • Hematopoietic stem/progenitor cells
  • Inflammation
  • Neurogenesis
  • Paracrine factor

ASJC Scopus subject areas

  • Immunology
  • Endocrine and Autonomic Systems
  • Behavioral Neuroscience

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