TY - JOUR
T1 - Circulating Progenitor Cell Response to Exercise in Wheelchair Racing Athletes
AU - Niemiro, Grace M.
AU - Edwards, Thomas
AU - Barfield, J. P.
AU - Beals, Joseph W.
AU - Broad, Elizabeth M.
AU - Motl, Robert W.
AU - Burd, Nicholas A.
AU - Pilutti, Lara A.
AU - De Lisio, Michael
N1 - Publisher Copyright:
© 2017 by the American College of Sports Medicine.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Introduction: Circulating progenitor cells (CPC) are a heterogeneous population of stem/progenitor cells in peripheral blood that participate in tissue repair. CPC mobilization has been well characterized in able-bodied persons but has not been previously investigated in wheelchair racing athletes. The purpose of this study was to characterize CPC and CPC subpopulation mobilization in elite wheelchair racing athletes in response to acute, upper-extremity aerobic exercise to determine whether CPC responses are similar to ambulatory populations. Methods: Eight participants (three females; age = 27.5 T 4.0 yr, supine height = 162.5 T 18.6 cm, weight = 53.5 T 10.9 kg, V? O2peak = 2.4 T 0.62 LIminj1, years postinjury = 21.5 T 6.2 yr) completed a 25-km time trial on a road course. Blood sampling occurred before and immediately after exercise for quantification of CPC (CD34+), hematopoietic stem and progenitor cells (HSPC) (CD34+/CD45dim), hematopoietic stem cells (HSC) (CD34+/CD45dim/CD38j), CD34+ adipose tissue (AT)-derived mesenchymal stromal cells (MSC) (CD45j/CD34+/CD105+/CD31j), CD34j bone marrow (BM)-derived MSC (CD45j/CD34j/CD105+/CD31j), and endothelial progenitor cells (EPC) (CD45dim/CD34+/VEGFR2+) via flow cytometry. Blood lactate was measured before and after trial as an indicator of exercise intensity. Results: CPC concentration increased 5.7-fold postexercise (P = 0.10). HSPC, HSC, EPC, and both MSC populations were not increased postexercise. Baseline HSPC populations were significantly positively correlated to absoluteV? O2peak (rho = 0.71, P G 0.05) with HSC trending to positively correlate to V? O2peak (rho = 0.62, P = 0.10). AT-MSC populations were trending to be negatively correlated to baseline V? O2peak (rho = j0.62, P = 0.058). The change in CPC, EPC, and AT-MSC pre- and postexercise significantly positively correlated to the change in lactate concentrations (rho = 0.91 P = 0.002, 0.71 P = 0.047, 0.81 P = 0.02, respectively, all P G 0.05). Conclusion: These data suggest that CPC content in wheelchair racing athletes is related to cardiorespiratory fitness, and responses to exercise are positively related to exercise intensity.
AB - Introduction: Circulating progenitor cells (CPC) are a heterogeneous population of stem/progenitor cells in peripheral blood that participate in tissue repair. CPC mobilization has been well characterized in able-bodied persons but has not been previously investigated in wheelchair racing athletes. The purpose of this study was to characterize CPC and CPC subpopulation mobilization in elite wheelchair racing athletes in response to acute, upper-extremity aerobic exercise to determine whether CPC responses are similar to ambulatory populations. Methods: Eight participants (three females; age = 27.5 T 4.0 yr, supine height = 162.5 T 18.6 cm, weight = 53.5 T 10.9 kg, V? O2peak = 2.4 T 0.62 LIminj1, years postinjury = 21.5 T 6.2 yr) completed a 25-km time trial on a road course. Blood sampling occurred before and immediately after exercise for quantification of CPC (CD34+), hematopoietic stem and progenitor cells (HSPC) (CD34+/CD45dim), hematopoietic stem cells (HSC) (CD34+/CD45dim/CD38j), CD34+ adipose tissue (AT)-derived mesenchymal stromal cells (MSC) (CD45j/CD34+/CD105+/CD31j), CD34j bone marrow (BM)-derived MSC (CD45j/CD34j/CD105+/CD31j), and endothelial progenitor cells (EPC) (CD45dim/CD34+/VEGFR2+) via flow cytometry. Blood lactate was measured before and after trial as an indicator of exercise intensity. Results: CPC concentration increased 5.7-fold postexercise (P = 0.10). HSPC, HSC, EPC, and both MSC populations were not increased postexercise. Baseline HSPC populations were significantly positively correlated to absoluteV? O2peak (rho = 0.71, P G 0.05) with HSC trending to positively correlate to V? O2peak (rho = 0.62, P = 0.10). AT-MSC populations were trending to be negatively correlated to baseline V? O2peak (rho = j0.62, P = 0.058). The change in CPC, EPC, and AT-MSC pre- and postexercise significantly positively correlated to the change in lactate concentrations (rho = 0.91 P = 0.002, 0.71 P = 0.047, 0.81 P = 0.02, respectively, all P G 0.05). Conclusion: These data suggest that CPC content in wheelchair racing athletes is related to cardiorespiratory fitness, and responses to exercise are positively related to exercise intensity.
KW - ENDOTHELIAL PROGENITOR CELL
KW - EXERCISE
KW - HEMATOPOIETIC STEM AND PROGENITOR CELLS
KW - MESENCHYMAL STEM CELL
KW - WHEELCHAIR RACING
UR - http://www.scopus.com/inward/record.url?scp=85038243975&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85038243975&partnerID=8YFLogxK
U2 - 10.1249/MSS.0000000000001402
DO - 10.1249/MSS.0000000000001402
M3 - Article
C2 - 28806276
AN - SCOPUS:85038243975
SN - 0195-9131
VL - 50
SP - 88
EP - 97
JO - Medicine and Science in Sports and Exercise
JF - Medicine and Science in Sports and Exercise
IS - 1
ER -