TY - JOUR
T1 - Circulating levels of transforming growth factor-β1 and lymphokines among children with hemolytic uremic syndrome
AU - Proulx, François
AU - Litalien, Catherine
AU - Turgeon, Jean P.
AU - Mariscalco, Michelle M.
AU - Seidman, Ernest
N1 - Funding Information:
Acknowledgment: The authors acknowledge the support of the Interservice Club Council (Telethon of Stars), granted to the “Groupe de Recherche Évaluative, Clinique et Épidémiologique,” Sainte-Justine Hospital (F.P.), to grant support from the Crohn's and Colitis Foundation of Canada (E.S.), and a research scholarship from the “Fonds de Recherche en Santé du Québec” (E.S.). We thank Elaine Orrbine and Peter McLaine from the Canadian Pediatric Kidney Disease Reference Centre and the Children's Hospital of Eastern Ontario for their support in recruitment of patients, the research assistants from the Sainte-Justine's Research Centre for their help in data collection, as well as Denise Lévesque and Anne Duhaime for their technical assistance.
PY - 2000
Y1 - 2000
N2 - Verotoxin-producing Escherichia coli (VTEC) cause hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). The aim of this study was to compare the circulating levels of transforming growth factor-β 1 (TGF-β1), T helper (T(H))1 (interferon [IFN]-γ, interleukin [IL]-2), and T(H)2- associated lymphokines (IL-4, IL-13) in children with uncomplicated Escherichia coli 0157:H7 HC and patients who developed HUS. Circulating levels of IL-2, IL-4, and IL-13 were undetectable, and those of IFN-γ, were low and comparable among groups. Concentrations of TGF-β1 were higher in children with uncomplicated 0157:H7 HC than among those who developed HUS (934 ± 680 versus 514 ± 497 pg/mL, respectively; P < 0.04). The circulating levels of TGF-β1 were also higher among children who did not take antidiarrheal agents (P < 0.008) and those who have been immediately discharged from the emergency room (P < 0.03). Our results did not show an imbalanced T(H)1/T(H)2-associated lymphokine response during the development of HUS. Increased circulating levels of TGF-β1 in children with milder 0157:H7 or uncomplicated HC most likely reflect appropriate intestinal tissue repair mechanisms rather than a remote systemic endocrine effect on the kidneys.
AB - Verotoxin-producing Escherichia coli (VTEC) cause hemorrhagic colitis (HC) and hemolytic uremic syndrome (HUS). The aim of this study was to compare the circulating levels of transforming growth factor-β 1 (TGF-β1), T helper (T(H))1 (interferon [IFN]-γ, interleukin [IL]-2), and T(H)2- associated lymphokines (IL-4, IL-13) in children with uncomplicated Escherichia coli 0157:H7 HC and patients who developed HUS. Circulating levels of IL-2, IL-4, and IL-13 were undetectable, and those of IFN-γ, were low and comparable among groups. Concentrations of TGF-β1 were higher in children with uncomplicated 0157:H7 HC than among those who developed HUS (934 ± 680 versus 514 ± 497 pg/mL, respectively; P < 0.04). The circulating levels of TGF-β1 were also higher among children who did not take antidiarrheal agents (P < 0.008) and those who have been immediately discharged from the emergency room (P < 0.03). Our results did not show an imbalanced T(H)1/T(H)2-associated lymphokine response during the development of HUS. Increased circulating levels of TGF-β1 in children with milder 0157:H7 or uncomplicated HC most likely reflect appropriate intestinal tissue repair mechanisms rather than a remote systemic endocrine effect on the kidneys.
KW - Child
KW - Cytokines
KW - Escherichia coli
KW - Hemolytic uremic syndrome (HUS)
KW - Interleukins (IL)
KW - Transforming growth factor (TGF)
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U2 - 10.1016/S0272-6386(00)70297-6
DO - 10.1016/S0272-6386(00)70297-6
M3 - Article
C2 - 10620540
AN - SCOPUS:0033966019
SN - 0272-6386
VL - 35
SP - 29
EP - 34
JO - American Journal of Kidney Diseases
JF - American Journal of Kidney Diseases
IS - 1
ER -