CircRNA-02191 regulating unsaturated fatty acid synthesis by adsorbing miR-145 to enhance CD36 expression in bovine mammary gland

Zhi Chen, Yuhao Wang, Kun Wang, Zhenbin Zhang, Mengli Han, Guoqing Li, Bin Zhang, Yang Yang, Juan J. Loor, Zhangping Yang, Fagang Zhong, Rong Dai, Mengzhi Wang

Research output: Contribution to journalArticlepeer-review


CD36 functions as a receptor for long-chain fatty acids, promoting the absorption and transport of long-chain unsaturated fatty acids. However, the regulatory influence of upstream circRNAs or miRNAs on its expression in cow mammary gland remains unclear. Herein, we performed high-throughput sequencing to screen for differentially expressed miRNAs and mRNAs in bovine mammary tissue during the late-lactation and the dry period to screen and conducted bioinformatics analysis to identify 420 miRNA/mRNA pairs, including miR-145/CD36. Experimental results indicate that miR-145 can directly target CD36 and inhibit its expression. Additionally, the circRNA-02191 sequence is predicted to contain a miR-145 binding site. As shown by dual luciferase reporter system detection, circRNA-02191 bound to miR-145 and its overexpression significantly reduced the expression of miR-145. Furthermore, the overexpression of miR-145 inhibited triglyceride accumulation, while circRNA-02191 enhanced the expression of the miR-145 target gene CD36. The above results indicate that circRNA-02191 can regulate triglyceride and fatty acid components by binding miR-145 and subsequently alleviating the inhibitory effect of miR-145 on the expression of CD36. Taken together, these findings present a novel approach to improve milk quality by analyzing the regulatory effect and mechanism regulating the circ02191/miR-145/CD36 pathway on fatty acid synthesis in the mammary gland of dairy cows.

Original languageEnglish (US)
Article number125306
JournalInternational Journal of Biological Macromolecules
StatePublished - Jul 31 2023


  • Bovine mammary gland
  • CD36
  • CircRNA-02191
  • MiR-145
  • Unsaturated fatty acid

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology
  • Biochemistry


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