Circadian rhythm of the rat suprachiasmatic brain slice is rapidly reset by daytime application of cAMP analogs

Martha Ulbrick Gillette; Rebecca A. Prosser

doi:10.1016/0006-8993(88)90449-0

Circadian rhythm of the rat suprachiasmatic brain slice is rapidly reset by daytime application of cAMP analogs

Martha Ulbrick Gillette, Rebecca A. Prosser

Research output: Contribution to journal › Article › peer-review

Abstract

Cellular mechanisms underlying the primary circadian pacemaker in mammals were investigated by isolating rat suprachiasmatic nuclei in brain slices and maintaining them in vitro for up to 3 days. The circadian rhythm of neuronal firing rate was used to assess the phase of the pacemaker. This rhythm was rapidly reset by bath application of cAMP analogs. Moreover, the pacemaker demonstrated circadian sensitivity to analog treatment: the rhythm was advanced by application during the donor's day, but not during the donor's night. These results suggest that cAMP-mediated events may stimulate pacemaker afferents within the SCN or may directly influence the pacemaker mechanism.

Original language	English (US)
Pages (from-to)	348-352
Number of pages	5
Journal	Brain Research
Volume	474
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(88)90449-0
State	Published - Dec 6 1988

Keywords

Brain slice
cAMP
Circadian rhythm
Hypothalamus
Oscillator
Pacemaker
Phase-shift
Suprachiasmatic nuclei

ASJC Scopus subject areas

Developmental Biology
Molecular Biology
Clinical Neurology
General Neuroscience

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10.1016/0006-8993(88)90449-0

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title = "Circadian rhythm of the rat suprachiasmatic brain slice is rapidly reset by daytime application of cAMP analogs",

abstract = "Cellular mechanisms underlying the primary circadian pacemaker in mammals were investigated by isolating rat suprachiasmatic nuclei in brain slices and maintaining them in vitro for up to 3 days. The circadian rhythm of neuronal firing rate was used to assess the phase of the pacemaker. This rhythm was rapidly reset by bath application of cAMP analogs. Moreover, the pacemaker demonstrated circadian sensitivity to analog treatment: the rhythm was advanced by application during the donor's day, but not during the donor's night. These results suggest that cAMP-mediated events may stimulate pacemaker afferents within the SCN or may directly influence the pacemaker mechanism.",

keywords = "Brain slice, cAMP, Circadian rhythm, Hypothalamus, Oscillator, Pacemaker, Phase-shift, Suprachiasmatic nuclei",

author = "Gillette, {Martha Ulbrick} and Prosser, {Rebecca A.}",

note = "The authors are indebted to Dr. Patricia DeCour-sey for her contributions to the development of 3-day culture of the SCN. We thank Drs. Rhanor Gillette, William Greenough, C. Ladd Prosser and Judith Willis for discussion and critical comments. Support of the National Institutes of Health (PHS Grant NS 22155) and the National Science Foundation (BNS Grant 84-19342) is gratefully acknowledged.",

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doi = "10.1016/0006-8993(88)90449-0",

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AU - Gillette, Martha Ulbrick

AU - Prosser, Rebecca A.

N1 - The authors are indebted to Dr. Patricia DeCour-sey for her contributions to the development of 3-day culture of the SCN. We thank Drs. Rhanor Gillette, William Greenough, C. Ladd Prosser and Judith Willis for discussion and critical comments. Support of the National Institutes of Health (PHS Grant NS 22155) and the National Science Foundation (BNS Grant 84-19342) is gratefully acknowledged.

PY - 1988/12/6

Y1 - 1988/12/6

N2 - Cellular mechanisms underlying the primary circadian pacemaker in mammals were investigated by isolating rat suprachiasmatic nuclei in brain slices and maintaining them in vitro for up to 3 days. The circadian rhythm of neuronal firing rate was used to assess the phase of the pacemaker. This rhythm was rapidly reset by bath application of cAMP analogs. Moreover, the pacemaker demonstrated circadian sensitivity to analog treatment: the rhythm was advanced by application during the donor's day, but not during the donor's night. These results suggest that cAMP-mediated events may stimulate pacemaker afferents within the SCN or may directly influence the pacemaker mechanism.

AB - Cellular mechanisms underlying the primary circadian pacemaker in mammals were investigated by isolating rat suprachiasmatic nuclei in brain slices and maintaining them in vitro for up to 3 days. The circadian rhythm of neuronal firing rate was used to assess the phase of the pacemaker. This rhythm was rapidly reset by bath application of cAMP analogs. Moreover, the pacemaker demonstrated circadian sensitivity to analog treatment: the rhythm was advanced by application during the donor's day, but not during the donor's night. These results suggest that cAMP-mediated events may stimulate pacemaker afferents within the SCN or may directly influence the pacemaker mechanism.

KW - Brain slice

KW - cAMP

KW - Circadian rhythm

KW - Hypothalamus

KW - Oscillator

KW - Pacemaker

KW - Phase-shift

KW - Suprachiasmatic nuclei

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ER -

Circadian rhythm of the rat suprachiasmatic brain slice is rapidly reset by daytime application of cAMP analogs

Abstract

Keywords

ASJC Scopus subject areas

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