Circadian clock gene expression regulates cancer cell growth through glutaminase

Aixia Huang, Bingbo Bao, H Rex Gaskins, Haijun Liu, Xueli Zhang, Liwen Lu, Shan Gao, Yihai Shi, Ming Zhang, Yuanzhou Shan, Jing Feng, Guoxiang Yao

Research output: Contribution to journalArticle

Abstract

Glutamine is an essential amino acid for malignant tumor cells. Glutaminase that metabolizes glutamine reaches a maximum expression in tumors immediately before the maximum proliferation rate. Tumor cells grow at different rates during the day. We postulated that the activity of glutaminase in tumor cells is subject to the regulation of circadian clock gene. We measured glutaminase by western blot analysis and circadian clock gene expression by real-time polymerase chain reaction in the liver and tumor cells at six equispaced time points of the day in individual mice of a 12/12 h light/dark schedule. The results showed that the tumor-bearing mice, under normal diurnal conditions, are circadianly entrained, as reflected by the normal host locomotor activity rhythms and rhythmic liver clock gene expression. The tumors within these mice are also circadianly organized, as reflected by circadian clock gene (Bmal1) expression. What is most remarkable is that kidney-type glutaminase also showed circadian rhythms in the same pattern with tumor circadian clock gene expression in liver cancer xenograft model, indicating that conditionally inhibiting glutaminase activity may provide a new target for cancer therapy.

Original languageEnglish (US)
Pages (from-to)409-414
Number of pages6
JournalActa Biochimica et Biophysica Sinica
Volume46
Issue number5
DOIs
StatePublished - Jan 1 2014

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Glutaminase
Circadian Clocks
Cell growth
Gene expression
Tumors
Clocks
Gene Expression
Growth
Neoplasms
Cells
Liver
Glutamine
Bearings (structural)
Essential Amino Acids
Polymerase chain reaction
Heterografts
Locomotion
Liver Neoplasms
Circadian Rhythm
Genes

Keywords

  • cancer
  • circadian clock gene
  • glutaminase

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

Cite this

Circadian clock gene expression regulates cancer cell growth through glutaminase. / Huang, Aixia; Bao, Bingbo; Gaskins, H Rex; Liu, Haijun; Zhang, Xueli; Lu, Liwen; Gao, Shan; Shi, Yihai; Zhang, Ming; Shan, Yuanzhou; Feng, Jing; Yao, Guoxiang.

In: Acta Biochimica et Biophysica Sinica, Vol. 46, No. 5, 01.01.2014, p. 409-414.

Research output: Contribution to journalArticle

Huang, A, Bao, B, Gaskins, HR, Liu, H, Zhang, X, Lu, L, Gao, S, Shi, Y, Zhang, M, Shan, Y, Feng, J & Yao, G 2014, 'Circadian clock gene expression regulates cancer cell growth through glutaminase', Acta Biochimica et Biophysica Sinica, vol. 46, no. 5, pp. 409-414. https://doi.org/10.1093/abbs/gmu012
Huang, Aixia ; Bao, Bingbo ; Gaskins, H Rex ; Liu, Haijun ; Zhang, Xueli ; Lu, Liwen ; Gao, Shan ; Shi, Yihai ; Zhang, Ming ; Shan, Yuanzhou ; Feng, Jing ; Yao, Guoxiang. / Circadian clock gene expression regulates cancer cell growth through glutaminase. In: Acta Biochimica et Biophysica Sinica. 2014 ; Vol. 46, No. 5. pp. 409-414.
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