Cilostamide affects in a concentration and exposure time-dependent manner the viability and the kinetics of in vitro maturation of caprine and bovine oocytes

H. H.V. Correia, L. A. Vieira, C. M. Mielgo, V. M. Paes, B. G. Alves, J. R.V. Silva, Matthew B Wheeler, A. P.R. Rodrigues, J. R. Figueiredo

Research output: Contribution to journalArticle

Abstract

This study investigated: 1) the kinetics of oocyte chromatin configuration during in vitro maturation (IVM) of caprine and bovine oocytes; and 2) the effect of in vitro pre-maturation (IVPM) with cilostamide with or without association of the follicular wall (FW) on the same parameters. In experiment I, cumulus-oocyte complexes (COCs) were cultured in vitro in a standard maturation medium for 6, 12, 18 or 30 h. For experiment II, the COCs were cultured for 30 h, either in a standard IVM medium or in IVPM containing cilostamide (10 or 20 μM) and FW alone or in combination, for 6 or 12 h before the onset of maturation. The MII rate was similar (P >.05) between 18 and 30 h of maturation, both of which were higher (P <.05) than 6 and 12 h IVM in both species (Experiment I). Contrary to caprine, all IVPM treatments presented a higher (P <.05) percentage of bovine oocytes arrested at the GV stage than the control treatment after 6 h of culture. The percentage of MII oocytes after 30 h (IVPM+IVM) of culture in bovine oocytes treated with 10 μM cilostamide associated with FW and FW alone cultured for 6 h presented MII percentages similar to the control. However, in caprine, these treatments significantly reduced the percentages of MII in relation to the control treatment (Experiment II). In conclusion, the combination of concentration-exposure time to cilostamide during IVPM delayed meiotic progression in bovine after 6 and 12 h of culture. However, overall the culture period (IVPM+IVM) influenced the oocyte chromatin configuration and kinetics in both species.

Original languageEnglish (US)
Pages (from-to)22-28
Number of pages7
JournalResearch in Veterinary Science
Volume122
DOIs
StatePublished - Feb 1 2019

Fingerprint

Oocytes
exposure duration
oocytes
goats
viability
kinetics
cattle
chromatin
Chromatin
cilostamide
In Vitro Techniques
In Vitro Oocyte Maturation Techniques
Therapeutics

Keywords

  • Bovine
  • Caprine
  • Cilostamide
  • Meiosis arrest
  • Pre-maturation

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Cilostamide affects in a concentration and exposure time-dependent manner the viability and the kinetics of in vitro maturation of caprine and bovine oocytes. / Correia, H. H.V.; Vieira, L. A.; Mielgo, C. M.; Paes, V. M.; Alves, B. G.; Silva, J. R.V.; Wheeler, Matthew B; Rodrigues, A. P.R.; Figueiredo, J. R.

In: Research in Veterinary Science, Vol. 122, 01.02.2019, p. 22-28.

Research output: Contribution to journalArticle

Correia, H. H.V. ; Vieira, L. A. ; Mielgo, C. M. ; Paes, V. M. ; Alves, B. G. ; Silva, J. R.V. ; Wheeler, Matthew B ; Rodrigues, A. P.R. ; Figueiredo, J. R. / Cilostamide affects in a concentration and exposure time-dependent manner the viability and the kinetics of in vitro maturation of caprine and bovine oocytes. In: Research in Veterinary Science. 2019 ; Vol. 122. pp. 22-28.
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T1 - Cilostamide affects in a concentration and exposure time-dependent manner the viability and the kinetics of in vitro maturation of caprine and bovine oocytes

AU - Correia, H. H.V.

AU - Vieira, L. A.

AU - Mielgo, C. M.

AU - Paes, V. M.

AU - Alves, B. G.

AU - Silva, J. R.V.

AU - Wheeler, Matthew B

AU - Rodrigues, A. P.R.

AU - Figueiredo, J. R.

PY - 2019/2/1

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N2 - This study investigated: 1) the kinetics of oocyte chromatin configuration during in vitro maturation (IVM) of caprine and bovine oocytes; and 2) the effect of in vitro pre-maturation (IVPM) with cilostamide with or without association of the follicular wall (FW) on the same parameters. In experiment I, cumulus-oocyte complexes (COCs) were cultured in vitro in a standard maturation medium for 6, 12, 18 or 30 h. For experiment II, the COCs were cultured for 30 h, either in a standard IVM medium or in IVPM containing cilostamide (10 or 20 μM) and FW alone or in combination, for 6 or 12 h before the onset of maturation. The MII rate was similar (P >.05) between 18 and 30 h of maturation, both of which were higher (P <.05) than 6 and 12 h IVM in both species (Experiment I). Contrary to caprine, all IVPM treatments presented a higher (P <.05) percentage of bovine oocytes arrested at the GV stage than the control treatment after 6 h of culture. The percentage of MII oocytes after 30 h (IVPM+IVM) of culture in bovine oocytes treated with 10 μM cilostamide associated with FW and FW alone cultured for 6 h presented MII percentages similar to the control. However, in caprine, these treatments significantly reduced the percentages of MII in relation to the control treatment (Experiment II). In conclusion, the combination of concentration-exposure time to cilostamide during IVPM delayed meiotic progression in bovine after 6 and 12 h of culture. However, overall the culture period (IVPM+IVM) influenced the oocyte chromatin configuration and kinetics in both species.

AB - This study investigated: 1) the kinetics of oocyte chromatin configuration during in vitro maturation (IVM) of caprine and bovine oocytes; and 2) the effect of in vitro pre-maturation (IVPM) with cilostamide with or without association of the follicular wall (FW) on the same parameters. In experiment I, cumulus-oocyte complexes (COCs) were cultured in vitro in a standard maturation medium for 6, 12, 18 or 30 h. For experiment II, the COCs were cultured for 30 h, either in a standard IVM medium or in IVPM containing cilostamide (10 or 20 μM) and FW alone or in combination, for 6 or 12 h before the onset of maturation. The MII rate was similar (P >.05) between 18 and 30 h of maturation, both of which were higher (P <.05) than 6 and 12 h IVM in both species (Experiment I). Contrary to caprine, all IVPM treatments presented a higher (P <.05) percentage of bovine oocytes arrested at the GV stage than the control treatment after 6 h of culture. The percentage of MII oocytes after 30 h (IVPM+IVM) of culture in bovine oocytes treated with 10 μM cilostamide associated with FW and FW alone cultured for 6 h presented MII percentages similar to the control. However, in caprine, these treatments significantly reduced the percentages of MII in relation to the control treatment (Experiment II). In conclusion, the combination of concentration-exposure time to cilostamide during IVPM delayed meiotic progression in bovine after 6 and 12 h of culture. However, overall the culture period (IVPM+IVM) influenced the oocyte chromatin configuration and kinetics in both species.

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KW - Caprine

KW - Cilostamide

KW - Meiosis arrest

KW - Pre-maturation

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