Chronic morphine alters the presynaptic protein profile: Identification of novel molecular targets using proteomics and network analysis

Noura S. Abul-Husn, Suresh P. Annangudi, Avi Ma'ayan, Dinah L. Ramos-Ortolaza, Steven D. Stockton, Ivone Gomes, Jonathan V. Sweedler, Lakshmi A. Devi

Research output: Contribution to journalArticle

Abstract

Opiates produce significant and persistent changes in synaptic transmission; knowledge of the proteins involved in these changes may help to understand the molecular mechanisms underlying opiate dependence. Using an integrated quantitative proteomics and systems biology approach, we explored changes in the presynaptic protein profile following a paradigm of chronic morphine administration that leads to the development of dependence. For this, we isolated presynaptic fractions from the striata of rats treated with saline or escalating doses of morphine, and analyzed the proteins in these fractions using differential isotopic labeling. We identified 30 proteins that were significantly altered by morphine and integrated them into a protein-protein interaction (PPI) network representing potential morphine-regulated protein complexes. Graph theory-based analysis of this network revealed clusters of densely connected and functionally related morphine-regulated clusters of proteins. One of the clusters contained molecular chaperones thought to be involved in regulation of neurotransmission. Within this cluster, cysteine-string protein (CSP) and the heat shock protein Hsc70 were downregulated by morphine. Interestingly, Hsp90, a heat shock protein that normally interacts with CSP and Hsc70, was upregulated by morphine. Moreover, treatment with the selective Hsp90 inhibitor, geldanamycin, decreased the somatic signs of naloxone-precipitated morphine withdrawal, suggesting that Hsp90 upregulation at the presynapse plays a role in the expression of morphine dependence. Thus, integration of proteomics, network analysis, and behavioral studies has provided a greater understanding of morphine-induced alterations in synaptic composition, and identified a potential novel therapeutic target for opiate dependence.

Original languageEnglish (US)
Article numbere25535
JournalPloS one
Volume6
Issue number10
DOIs
StatePublished - Oct 17 2011

Fingerprint

morphine
Electric network analysis
Proteomics
proteomics
Morphine
Opiate Alkaloids
Proteins
proteins
Opioid-Related Disorders
Heat-Shock Proteins
Synaptic Transmission
heat shock proteins
cysteine
Morphine Dependence
Protein Interaction Maps
Molecular Chaperones
Systems Biology
naloxone
synaptic transmission
molecular chaperones

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)
  • General

Cite this

Abul-Husn, N. S., Annangudi, S. P., Ma'ayan, A., Ramos-Ortolaza, D. L., Stockton, S. D., Gomes, I., ... Devi, L. A. (2011). Chronic morphine alters the presynaptic protein profile: Identification of novel molecular targets using proteomics and network analysis. PloS one, 6(10), [e25535]. https://doi.org/10.1371/journal.pone.0025535

Chronic morphine alters the presynaptic protein profile : Identification of novel molecular targets using proteomics and network analysis. / Abul-Husn, Noura S.; Annangudi, Suresh P.; Ma'ayan, Avi; Ramos-Ortolaza, Dinah L.; Stockton, Steven D.; Gomes, Ivone; Sweedler, Jonathan V.; Devi, Lakshmi A.

In: PloS one, Vol. 6, No. 10, e25535, 17.10.2011.

Research output: Contribution to journalArticle

Abul-Husn, Noura S. ; Annangudi, Suresh P. ; Ma'ayan, Avi ; Ramos-Ortolaza, Dinah L. ; Stockton, Steven D. ; Gomes, Ivone ; Sweedler, Jonathan V. ; Devi, Lakshmi A. / Chronic morphine alters the presynaptic protein profile : Identification of novel molecular targets using proteomics and network analysis. In: PloS one. 2011 ; Vol. 6, No. 10.
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