Chronic exposure of a freshwater mussel to elevated pCO 2: Effects on the control of biomineralization and ion-regulatory responses

Jennifer D. Jeffrey, Kelly D. Hannan, Caleb T. Hasler, Cory D. Suski

Research output: Contribution to journalArticle

Abstract

Freshwater mussels may be exposed to elevations in mean partial pressure of carbon dioxide (pCO 2 ) caused by both natural and anthropogenic factors. The goal of the present study was to assess the effects of a 28-d elevation in pCO 2 at 15 000 and 50 000 μatm on processes associated with biomineralization, ion regulation, and cellular stress in adult Lampsilis siliquoidea (Barnes, 1823). In addition, the capacity for mussels to compensate for acid-base disturbances experienced after exposure to elevated pCO 2 was assessed over a 14-d recovery period. Overall, exposure to 50 000 μatm pCO 2 had more pronounced physiological consequences compared with 15 000 μatm pCO 2 . Over the first 7 d of exposure to 50 000 μatm pCO 2 , the mRNA abundance of chitin synthase (cs), calmodulin (cam), and calmodulin-like protein (calp) were significantly affected, suggesting that shell formation and integrity may be altered during pCO 2 exposure. After the removal of the pCO 2 treatment, mussels may compensate for the acid-base and ion disturbances experienced during pCO 2 exposure, and transcript levels of some regulators of biomineralization (carbonic anhydrase [ca], cs, cam, calp) as well as ion regulation (na + -k + -ATPase [nka]) were modulated. Effects of elevated pCO 2 on heat shock protein 70 (hsp70) were limited in the present study. Overall, adult L. siliquoidea appeared to regulate factors associated with the control of biomineralization and ion regulation during and/or after the removal of pCO 2 exposure. Environ Toxicol Chem 2018;37:538–550.

Original languageEnglish (US)
Pages (from-to)538-550
Number of pages13
JournalEnvironmental Toxicology and Chemistry
Volume37
Issue number2
DOIs
StatePublished - Feb 1 2018

Fingerprint

Biomineralization
biomineralization
Bivalvia
Calmodulin
Fresh Water
Chitin Synthase
Ions
ion
chitin
HSP70 Heat-Shock Proteins
Acids
protein
Carbonic Anhydrases
Partial Pressure
Carbon Dioxide
Partial pressure
Adenosine Triphosphatases
disturbance
Proteins
acid

Keywords

  • Benthic macroinvertebrates
  • Calmodulin
  • Chitin synthase
  • Freshwater toxicology
  • Mollusk toxicology
  • Na -K -ATPase

ASJC Scopus subject areas

  • Environmental Chemistry
  • Health, Toxicology and Mutagenesis

Cite this

Chronic exposure of a freshwater mussel to elevated pCO 2 : Effects on the control of biomineralization and ion-regulatory responses. / Jeffrey, Jennifer D.; Hannan, Kelly D.; Hasler, Caleb T.; Suski, Cory D.

In: Environmental Toxicology and Chemistry, Vol. 37, No. 2, 01.02.2018, p. 538-550.

Research output: Contribution to journalArticle

@article{9c7fa55c78f04428906239b7fd382780,
title = "Chronic exposure of a freshwater mussel to elevated pCO 2: Effects on the control of biomineralization and ion-regulatory responses",
abstract = "Freshwater mussels may be exposed to elevations in mean partial pressure of carbon dioxide (pCO 2 ) caused by both natural and anthropogenic factors. The goal of the present study was to assess the effects of a 28-d elevation in pCO 2 at 15 000 and 50 000 μatm on processes associated with biomineralization, ion regulation, and cellular stress in adult Lampsilis siliquoidea (Barnes, 1823). In addition, the capacity for mussels to compensate for acid-base disturbances experienced after exposure to elevated pCO 2 was assessed over a 14-d recovery period. Overall, exposure to 50 000 μatm pCO 2 had more pronounced physiological consequences compared with 15 000 μatm pCO 2 . Over the first 7 d of exposure to 50 000 μatm pCO 2 , the mRNA abundance of chitin synthase (cs), calmodulin (cam), and calmodulin-like protein (calp) were significantly affected, suggesting that shell formation and integrity may be altered during pCO 2 exposure. After the removal of the pCO 2 treatment, mussels may compensate for the acid-base and ion disturbances experienced during pCO 2 exposure, and transcript levels of some regulators of biomineralization (carbonic anhydrase [ca], cs, cam, calp) as well as ion regulation (na + -k + -ATPase [nka]) were modulated. Effects of elevated pCO 2 on heat shock protein 70 (hsp70) were limited in the present study. Overall, adult L. siliquoidea appeared to regulate factors associated with the control of biomineralization and ion regulation during and/or after the removal of pCO 2 exposure. Environ Toxicol Chem 2018;37:538–550.",
keywords = "Benthic macroinvertebrates, Calmodulin, Chitin synthase, Freshwater toxicology, Mollusk toxicology, Na -K -ATPase",
author = "Jeffrey, {Jennifer D.} and Hannan, {Kelly D.} and Hasler, {Caleb T.} and Suski, {Cory D.}",
year = "2018",
month = "2",
day = "1",
doi = "10.1002/etc.3991",
language = "English (US)",
volume = "37",
pages = "538--550",
journal = "Environmental Toxicology and Chemistry",
issn = "0730-7268",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

TY - JOUR

T1 - Chronic exposure of a freshwater mussel to elevated pCO 2

T2 - Effects on the control of biomineralization and ion-regulatory responses

AU - Jeffrey, Jennifer D.

AU - Hannan, Kelly D.

AU - Hasler, Caleb T.

AU - Suski, Cory D.

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Freshwater mussels may be exposed to elevations in mean partial pressure of carbon dioxide (pCO 2 ) caused by both natural and anthropogenic factors. The goal of the present study was to assess the effects of a 28-d elevation in pCO 2 at 15 000 and 50 000 μatm on processes associated with biomineralization, ion regulation, and cellular stress in adult Lampsilis siliquoidea (Barnes, 1823). In addition, the capacity for mussels to compensate for acid-base disturbances experienced after exposure to elevated pCO 2 was assessed over a 14-d recovery period. Overall, exposure to 50 000 μatm pCO 2 had more pronounced physiological consequences compared with 15 000 μatm pCO 2 . Over the first 7 d of exposure to 50 000 μatm pCO 2 , the mRNA abundance of chitin synthase (cs), calmodulin (cam), and calmodulin-like protein (calp) were significantly affected, suggesting that shell formation and integrity may be altered during pCO 2 exposure. After the removal of the pCO 2 treatment, mussels may compensate for the acid-base and ion disturbances experienced during pCO 2 exposure, and transcript levels of some regulators of biomineralization (carbonic anhydrase [ca], cs, cam, calp) as well as ion regulation (na + -k + -ATPase [nka]) were modulated. Effects of elevated pCO 2 on heat shock protein 70 (hsp70) were limited in the present study. Overall, adult L. siliquoidea appeared to regulate factors associated with the control of biomineralization and ion regulation during and/or after the removal of pCO 2 exposure. Environ Toxicol Chem 2018;37:538–550.

AB - Freshwater mussels may be exposed to elevations in mean partial pressure of carbon dioxide (pCO 2 ) caused by both natural and anthropogenic factors. The goal of the present study was to assess the effects of a 28-d elevation in pCO 2 at 15 000 and 50 000 μatm on processes associated with biomineralization, ion regulation, and cellular stress in adult Lampsilis siliquoidea (Barnes, 1823). In addition, the capacity for mussels to compensate for acid-base disturbances experienced after exposure to elevated pCO 2 was assessed over a 14-d recovery period. Overall, exposure to 50 000 μatm pCO 2 had more pronounced physiological consequences compared with 15 000 μatm pCO 2 . Over the first 7 d of exposure to 50 000 μatm pCO 2 , the mRNA abundance of chitin synthase (cs), calmodulin (cam), and calmodulin-like protein (calp) were significantly affected, suggesting that shell formation and integrity may be altered during pCO 2 exposure. After the removal of the pCO 2 treatment, mussels may compensate for the acid-base and ion disturbances experienced during pCO 2 exposure, and transcript levels of some regulators of biomineralization (carbonic anhydrase [ca], cs, cam, calp) as well as ion regulation (na + -k + -ATPase [nka]) were modulated. Effects of elevated pCO 2 on heat shock protein 70 (hsp70) were limited in the present study. Overall, adult L. siliquoidea appeared to regulate factors associated with the control of biomineralization and ion regulation during and/or after the removal of pCO 2 exposure. Environ Toxicol Chem 2018;37:538–550.

KW - Benthic macroinvertebrates

KW - Calmodulin

KW - Chitin synthase

KW - Freshwater toxicology

KW - Mollusk toxicology

KW - Na -K -ATPase

UR - http://www.scopus.com/inward/record.url?scp=85037979667&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85037979667&partnerID=8YFLogxK

U2 - 10.1002/etc.3991

DO - 10.1002/etc.3991

M3 - Article

C2 - 28971536

AN - SCOPUS:85037979667

VL - 37

SP - 538

EP - 550

JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

IS - 2

ER -