Chromosome-specific microsatellite multiplex sets for linkage studies in the domestic dog

Leigh Anne Clark, Kate L. Tsai, Jörg M. Steiner, David A. Williams, Trina Guerra, Elaine A. Ostrander, Francis Galibert, Keith E. Murphy

Research output: Contribution to journalArticlepeer-review

Abstract

To expedite linkage studies and positional cloning efforts in the dog, Minimal Screening Set 2 (MSS-2) of 327 canine microsatellite markers has been multiplexed into chromosome-specific panels. MSS-2 provides 9 Mb coverage of the canine genome with no gaps larger than 17.1 Mb and is the most recent and comprehensive set of microsatellites available for whole-genome scans. Markers were labeled with fluorescent dyes based on locations and expected product sizes to facilitate the multiplexing of a maximum number of markers for each chromosome. All markers are amplified using a single thermal cycling program and PCR mix and are optimized for resolution on an ABI 3100 genetic analyzer. Sixty-nine chromosome-specific panels were created by coamplification of a maximum number of markers and subsequent coloading of the remaining markers.

Original languageEnglish (US)
Pages (from-to)550-554
Number of pages5
JournalGenomics
Volume84
Issue number3
DOIs
StatePublished - Sep 1 2004
Externally publishedYes

Keywords

  • Chromosome-specific
  • Dog
  • Linkage
  • Microsatellite
  • Multiplexing

ASJC Scopus subject areas

  • Genetics

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