Chromosome Nomenclature and Cytological Characterization of Sacred Lotus

Zhuang Meng, Xiaoxu Hu, Zhiliang Zhang, Zhanjie Li, Qingfang Lin, Mei Yang, Pingfang Yang, Ray Ming, Qingyi Yu, Kai Wang

Research output: Contribution to journalArticle

Abstract

Sacred lotus is a basal eudicot plant that has been cultivated in Asia for over 7,000 years for its agricultural, ornamental, religious, and medicinal importance. A notable characteristic of lotus is the seed longevity. Extensive endeavors have been devoted to dissect its genome assembly, including the variety China Antique, which germinated from a 1,300-year-old seed. Here, cytogenetic markers representing the 10 largest megascaffolds, which constitute approximately 70% of the lotus genome assembly, were developed. These 10 megascaffolds were then anchored to the corresponding lotus chromosomes by fluorescence in situ hybridization using these cytogenetic markers, and a set of chromosome-specific cytogenetic markers that could unambiguously identify each of the 8 chromosomes was generated. Karyotyping was conducted, and a nomenclature based on chromosomal length was established for the 8 chromosomes of China Antique. Comparative karyotyping revealed relatively conserved chromosomal structures between China Antique and 3 modern cultivars. Interestingly, significant variations in the copy number of 45S rDNA were detected between China Antique and modern cultivars. Our results provide a comprehensive view on the chromosomal structure of sacred lotus and will facilitate further studies and the genome assembly of lotus.

Original languageEnglish (US)
Pages (from-to)223-231
Number of pages9
JournalCytogenetic and Genome Research
Volume153
Issue number4
DOIs
StatePublished - Mar 1 2018

Keywords

  • Chromosome nomenclature
  • Cytological marker
  • Fluorescence in situ hybridization
  • Megascaffold
  • Sacred lotus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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  • Cite this

    Meng, Z., Hu, X., Zhang, Z., Li, Z., Lin, Q., Yang, M., Yang, P., Ming, R., Yu, Q., & Wang, K. (2018). Chromosome Nomenclature and Cytological Characterization of Sacred Lotus. Cytogenetic and Genome Research, 153(4), 223-231. https://doi.org/10.1159/000486777