Chromosome Nomenclature and Cytological Characterization of Sacred Lotus

Zhuang Meng, Xiaoxu Hu, Zhiliang Zhang, Zhanjie Li, Qingfang Lin, Mei Yang, Pingfang Yang, Ray Ming, Qingyi Yu, Kai Wang

Abstract

Sacred lotus is a basal eudicot plant that has been cultivated in Asia for over 7,000 years for its agricultural, ornamental, religious, and medicinal importance. A notable characteristic of lotus is the seed longevity. Extensive endeavors have been devoted to dissect its genome assembly, including the variety China Antique, which germinated from a 1,300-year-old seed. Here, cytogenetic markers representing the 10 largest megascaffolds, which constitute approximately 70% of the lotus genome assembly, were developed. These 10 megascaffolds were then anchored to the corresponding lotus chromosomes by fluorescence in situ hybridization using these cytogenetic markers, and a set of chromosome-specific cytogenetic markers that could unambiguously identify each of the 8 chromosomes was generated. Karyotyping was conducted, and a nomenclature based on chromosomal length was established for the 8 chromosomes of China Antique. Comparative karyotyping revealed relatively conserved chromosomal structures between China Antique and 3 modern cultivars. Interestingly, significant variations in the copy number of 45S rDNA were detected between China Antique and modern cultivars. Our results provide a comprehensive view on the chromosomal structure of sacred lotus and will facilitate further studies and the genome assembly of lotus.

Original languageEnglish (US)
Pages (from-to)223-231
Number of pages9
JournalCytogenetic and Genome Research
Volume153
Issue number4
DOIs
StatePublished - Mar 1 2018

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Keywords

  • Chromosome nomenclature
  • Cytological marker
  • Fluorescence in situ hybridization
  • Megascaffold
  • Sacred lotus

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Meng, Z., Hu, X., Zhang, Z., Li, Z., Lin, Q., Yang, M., Yang, P., Ming, R., Yu, Q., & Wang, K. (2018). Chromosome Nomenclature and Cytological Characterization of Sacred Lotus. Cytogenetic and Genome Research, 153(4), 223-231. https://doi.org/10.1159/000486777