Chromogenic assay for phospholipase C from bacillus cereus

Paul J. Hergenrother, Mark R. Spaller, Michelle K. Haas, Stephen F. Martin

Research output: Contribution to journalArticlepeer-review

Abstract

A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (Km and Vmax) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc). The assay is applied to the determination of the Ki for a known inhibitor of PLCBc.

Original languageEnglish (US)
Pages (from-to)313-316
Number of pages4
JournalAnalytical Biochemistry
Volume229
Issue number2
DOIs
StatePublished - Aug 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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