Chromogenic assay for phospholipase C from bacillus cereus

Paul J. Hergenrother; Mark R. Spaller; Michelle K. Haas; Stephen F. Martin

doi:10.1006/abio.1995.1418

Chromogenic assay for phospholipase C from bacillus cereus

Paul J. Hergenrother, Mark R. Spaller, Michelle K. Haas, Stephen F. Martin

Research output: Contribution to journal › Article › peer-review

Abstract

A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (K_m and V_max) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLC_Bc). The assay is applied to the determination of the K_i for a known inhibitor of PLC_Bc.

Original language	English (US)
Pages (from-to)	313-316
Number of pages	4
Journal	Analytical Biochemistry
Volume	229
Issue number	2
DOIs	https://doi.org/10.1006/abio.1995.1418
State	Published - Aug 1995
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1006/abio.1995.1418

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title = "Chromogenic assay for phospholipase C from bacillus cereus",

abstract = "A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (Km and Vmax) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc). The assay is applied to the determination of the Ki for a known inhibitor of PLCBc.",

author = "Hergenrother, {Paul J.} and Spaller, {Mark R.} and Haas, {Michelle K.} and Martin, {Stephen F.}",

year = "1995",

month = aug,

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journal = "Analytical Biochemistry",

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AU - Spaller, Mark R.

AU - Haas, Michelle K.

AU - Martin, Stephen F.

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N2 - A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (Km and Vmax) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc). The assay is applied to the determination of the Ki for a known inhibitor of PLCBc.

AB - A standard chromogenic assay for phosphatidylcholine determination has been modified in the development of a facile method for measuring the rate parameters (Km and Vmax) for phosphatidylcholine-preferring phospholipase C from Bacillus cereus (PLCBc). The assay is applied to the determination of the Ki for a known inhibitor of PLCBc.

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JF - Analytical Biochemistry

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Chromogenic assay for phospholipase C from bacillus cereus

Abstract

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