Chromatin’s Influence on Pre-Replication Complex Assembly and Function

Hina Ahmad, Neha Chetlangia, Supriya G. Prasanth

Research output: Contribution to journalReview articlepeer-review


In all eukaryotes, the initiation of DNA replication requires a stepwise assembly of factors onto the origins of DNA replication. This is pioneered by the Origin Recognition Complex, which recruits Cdc6. Together, they bring Cdt1, which shepherds MCM2-7 to form the OCCM complex. Sequentially, a second Cdt1-bound hexamer of MCM2-7 is recruited by ORC-Cdc6 to form an MCM double hexamer, which forms a part of the pre-RC. Although the mechanism of ORC binding to DNA varies across eukaryotes, how ORC is recruited to replication origins in human cells remains an area of intense investigation. This review discusses how the chromatin environment influences pre-RC assembly, function, and, eventually, origin activity.

Original languageEnglish (US)
Article number152
Issue number3
StatePublished - Mar 2024


  • DNA replication
  • polycomb (Pc)
  • epigenetics
  • pre-replication complex (pre-RC)
  • ORC
  • chromatin

ASJC Scopus subject areas

  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • General Agricultural and Biological Sciences


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