Chlorate transport in isolated tonoplast vesicles from red beet (Beta vulgaris L.) storage tissue

Amy J. Chodera, Donald P Briskin

Research output: Contribution to journalArticle

Abstract

There is strong evidence that 36ClO3- can serve as a suitable radiotracer for nitrate in transport experiments with plant tissue. In this study, 36ClO3- was used to investigate the mechanism of nitrate influx and efflux in sealed tonoplast membrane vesicles isolated from red beet (Beta vulgaris L. cv. Detroit Dark Red) storage tissue. Uptake of 36ClO3- into red beet tonoplast vesicles was ATP-dependent, inhibited by carbonylcyanide m-chlorophenylhydrazone and demonstrated saturation kinetics with a Km of about 5 mM. While ATP-driven 36ClO3- uptake was inhibited by the collapse of the membrane electrical potential (ΔΨ) and an artificially imposed pH gradient could not drive radiolabel uptake, radiolabel uptake could be driven by an artificially imposed, positive-interior ΔΨ. These results suggest that ATP-driven 36ClO3- influx at the tonoplast may be mediated by a uniport system which responds to the positive interior membrane potential. When 36ClO3- was pre-loaded into the vesicles and radiolabel efflux was examined, no evidence could be found for the existence of an ATP-driven H+/36ClO3- symport for efflux. Overall, this study demonstrates the feasibility of using 36ClO3- as a radiotracer with isolated membrane vesicles and sets the stage for further research on the mechanism of NO3- transport systems.

Original languageEnglish (US)
Pages (from-to)151-160
Number of pages10
JournalPlant Science
Volume67
Issue number2
DOIs
StatePublished - 1990

Fingerprint

Chlorates
chlorates
Beta vulgaris
tonoplast
beets
Adenosine Triphosphate
uptake mechanisms
Ion Transport
Nitrates
Membrane Potentials
nitrates
Proton-Motive Force
Membranes
Feasibility Studies
membrane potential
plant tissues
kinetics
tissues
Research

Keywords

  • membrane transport systems
  • nitrate transport
  • proton electrochemical gradient
  • radiotracer methodology

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

Cite this

Chlorate transport in isolated tonoplast vesicles from red beet (Beta vulgaris L.) storage tissue. / Chodera, Amy J.; Briskin, Donald P.

In: Plant Science, Vol. 67, No. 2, 1990, p. 151-160.

Research output: Contribution to journalArticle

Chodera, Amy J. ; Briskin, Donald P. / Chlorate transport in isolated tonoplast vesicles from red beet (Beta vulgaris L.) storage tissue. In: Plant Science. 1990 ; Vol. 67, No. 2. pp. 151-160.
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