Chimpanzee and pig-tailed macaque iPSCs: Improved culture and generation of primate cross-species embryos

Morteza Roodgar, Fabian P. Suchy, Lan H. Nguyen, Vivek K. Bajpai, Rahul Sinha, Jose G. Vilches-Moure, Kevin Van Bortle, Joydeep Bhadury, Ahmed Metwally, Lihua Jiang, Ruiqi Jian, Rosaria Chiang, Angelos Oikonomopoulos, Joseph C. Wu, Irving L. Weissman, Joseph L. Mankowski, Susan Holmes, Kyle M. Loh, Hiromitsu Nakauchi, Catherine A. VandeVoortMichael P. Snyder

Research output: Contribution to journalArticlepeer-review


As our closest living relatives, non-human primates uniquely enable explorations of human health, disease, development, and evolution. Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. Here, we establish improved culture methods for chimpanzee (Pan troglodytes) and pig-tailed macaque (Macaca nemestrina) iPSCs. Such iPSCs spontaneously differentiate in conventional culture conditions, but can be readily propagated by inhibiting endogenous WNT signaling. As a unique functional test of these iPSCs, we injected them into the pre-implantation embryos of another non-human species, rhesus macaques (Macaca mulatta). Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research.

Original languageEnglish (US)
Article number111264
JournalCell Reports
Issue number9
StatePublished - Aug 30 2022
Externally publishedYes


  • CP: Developmental biology
  • Nonhuman primates
  • blastocyst, BCL2
  • chimpanzee
  • cross-species embryos
  • iPSCs
  • interspecies chimera
  • pig-tailed macaque

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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