Chimeric single-stranded DNA phage-plasmid cloning vectors.

D. A. Mead, B. Kemper

Research output: Contribution to journalReview article

Abstract

A variety of ssDNA phagemid cloning vectors have been constructed that combine the advantages of the filamentous coliphages with a number of plasmid and other bacteriophage-encoded functions. The practical and biological advantages of a chimeric phage-plasmid vector are considerable, and the trend toward converting existing plasmids into ssDNA phagemids and consolidating any number of useful features into one or a few vectors will undoubtedly accelerate. A new helper phage specifically designed for the isolation of large amounts of single-stranded plasmid DNA simplifies the use of these cloning vehicles. Additional refinements in phagemid-helper phage systems should extend the potential of these vectors even further.

Original languageEnglish (US)
Pages (from-to)85-102
Number of pages18
JournalBiotechnology (Reading, Mass.)
Volume10
DOIs
StatePublished - 1988
Externally publishedYes

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