TY - JOUR
T1 - Chia (Salvia hispanica L.) Seed Total Protein and Protein Fractions Digests Reduce Biomarkers of Inflammation and Atherosclerosis in Macrophages In Vitro
AU - Grancieri, Mariana
AU - Martino, Hercia Stampini Duarte
AU - Gonzalez de Mejia, Elvira
N1 - M.G. executed the project and prepared the initial draft; H.S.D.M. perfomed critical analysis; E.G.M. did overall concept, scientific guidance throughout the research, editing of the manuscript, and critical analysis. All authors critically revised the manuscript and gave final approval for submission. Conselho Nacional de Ciência e Tecnologia Brasileira (CNPq), Brazil for MG's scholarship (Grant No. 200739/2017‐4) and HSDM Research Productivity's fellowships are acknowledged. This study was financed in part by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Brazil (CAPES) (Grant No. 001) and ACES International Joint Research Program, University of Illinois (research was supported by the USDA‐NIFA‐HATCH project 1014457).
M.G. executed the project and prepared the initial draft; H.S.D.M. perfomed critical analysis; E.G.M. did overall concept, scientific guidance throughout the research, editing of the manuscript, and critical analysis. All authors critically revised the manuscript and gave final approval for submission. Conselho Nacional de Ci?ncia e Tecnologia Brasileira (CNPq), Brazil for MG's scholarship (Grant No. 200739/2017-4) and HSDM Research Productivity's fellowships are acknowledged. This study was financed in part by Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, Brazil (CAPES) (Grant No. 001) and ACES International Joint Research Program, University of Illinois (research was supported by the USDA-NIFA-HATCH project 1014457).
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Scope: The objectives are to evaluate the anti-inflammatory and anti-atherosclerotic effects of digested total protein and digested protein fractions from chia seed in macrophages in vitro. Methods and results: Total protein and protein fractions (albumin, globulin, glutelin, and prolamin) are isolated from chia seed and digested using simulated gastrointestinal conditions, resulting in digested total protein (DTP) and digested protein fractions (DPF). DTP and DPF are applied (1.0 mg mL−1) in RAW 264.4 macrophages stimulated with LPS (1 µg mL−1) for inflammation or ox-LDL (80 µg mL−1) for atherosclerosis. In the inflammatory process, DTP and DPF reduce p-NF-κB, iNOS, p-JNK, and AP-1. Digested glutelin reduces the secretion of nitric oxide (65.1%), reactive oxygen species (19.7%), prostaglandins (34.6%), TNF-α (24.1%), MCP-1 (18.9%), IL-6 (39.6%), and IL-10 (68.7%). DTP and DPF reduce the NF-κB translocation to nuclei. DTP and digested glutelin reduce iCAM expression (86.4%, 80.8%), LOX-1 (37.3%, 35.7%), iNOS (67.0%, 42.2%), and NF-κB (57.5%, 71.1%). DTP is effective in reducing secretion of nitric oxide (43.4%), lipid accumulation (41.9%), prostaglandins (41.9%), TNF-α (43.3%), MCP-1 (47.6%), and IL-6 (50.5%). Peptides from chia DTP and DPF are also characterized. Conclusion: DTP and digested glutelin from chia seed reduce expression and secretion of markers related to inflammation and atherosclerosis pathways.
AB - Scope: The objectives are to evaluate the anti-inflammatory and anti-atherosclerotic effects of digested total protein and digested protein fractions from chia seed in macrophages in vitro. Methods and results: Total protein and protein fractions (albumin, globulin, glutelin, and prolamin) are isolated from chia seed and digested using simulated gastrointestinal conditions, resulting in digested total protein (DTP) and digested protein fractions (DPF). DTP and DPF are applied (1.0 mg mL−1) in RAW 264.4 macrophages stimulated with LPS (1 µg mL−1) for inflammation or ox-LDL (80 µg mL−1) for atherosclerosis. In the inflammatory process, DTP and DPF reduce p-NF-κB, iNOS, p-JNK, and AP-1. Digested glutelin reduces the secretion of nitric oxide (65.1%), reactive oxygen species (19.7%), prostaglandins (34.6%), TNF-α (24.1%), MCP-1 (18.9%), IL-6 (39.6%), and IL-10 (68.7%). DTP and DPF reduce the NF-κB translocation to nuclei. DTP and digested glutelin reduce iCAM expression (86.4%, 80.8%), LOX-1 (37.3%, 35.7%), iNOS (67.0%, 42.2%), and NF-κB (57.5%, 71.1%). DTP is effective in reducing secretion of nitric oxide (43.4%), lipid accumulation (41.9%), prostaglandins (41.9%), TNF-α (43.3%), MCP-1 (47.6%), and IL-6 (50.5%). Peptides from chia DTP and DPF are also characterized. Conclusion: DTP and digested glutelin from chia seed reduce expression and secretion of markers related to inflammation and atherosclerosis pathways.
KW - Salvia hispanica L
KW - atherosclerosis
KW - bioactive peptides
KW - digestion
KW - inflammation
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U2 - 10.1002/mnfr.201900021
DO - 10.1002/mnfr.201900021
M3 - Article
C2 - 31343098
AN - SCOPUS:85070672692
SN - 1613-4125
VL - 63
JO - Molecular Nutrition and Food Research
JF - Molecular Nutrition and Food Research
IS - 19
M1 - 1900021
ER -