Abstract
Conditions for assaying chemotaxis in B. subtilis are described. The chemotaxis medium that was used afforded excellent motility for hours. In it, chemotaxis measured by capillary assays was insensitive to pH between 5.5 and 9, and to temperature between 28°C and 42°C. Chemotaxis was observed toward all 20 common amino acids, with thresholds varying from 3 nM for alanine to 0.1 mM for glutamate, in the capillary assay, and from 0.1 μM for alanine to 0.32 mM for glutamate in the microscope assay.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 151-155 |
| Number of pages | 5 |
| Journal | Journal of Bacteriology |
| Volume | 129 |
| Issue number | 1 |
| State | Published - 1977 |
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ASJC Scopus subject areas
- Microbiology
- Molecular Biology
Cite this
Chemotaxis toward amino acids by Bacillus subtilis. / Ordal, George W; Gibson, K. J.
In: Journal of Bacteriology, Vol. 129, No. 1, 1977, p. 151-155.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Chemotaxis toward amino acids by Bacillus subtilis
AU - Ordal, George W
AU - Gibson, K. J.
PY - 1977
Y1 - 1977
N2 - Conditions for assaying chemotaxis in B. subtilis are described. The chemotaxis medium that was used afforded excellent motility for hours. In it, chemotaxis measured by capillary assays was insensitive to pH between 5.5 and 9, and to temperature between 28°C and 42°C. Chemotaxis was observed toward all 20 common amino acids, with thresholds varying from 3 nM for alanine to 0.1 mM for glutamate, in the capillary assay, and from 0.1 μM for alanine to 0.32 mM for glutamate in the microscope assay.
AB - Conditions for assaying chemotaxis in B. subtilis are described. The chemotaxis medium that was used afforded excellent motility for hours. In it, chemotaxis measured by capillary assays was insensitive to pH between 5.5 and 9, and to temperature between 28°C and 42°C. Chemotaxis was observed toward all 20 common amino acids, with thresholds varying from 3 nM for alanine to 0.1 mM for glutamate, in the capillary assay, and from 0.1 μM for alanine to 0.32 mM for glutamate in the microscope assay.
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UR - http://www.scopus.com/inward/citedby.url?scp=0017344854&partnerID=8YFLogxK
M3 - Article
C2 - 12134
AN - SCOPUS:0017344854
VL - 129
SP - 151
EP - 155
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 1
ER -