Chemotaxis toward amino acids by Bacillus subtilis

G. W. Ordal, K. J. Gibson

Research output: Contribution to journalArticle

Abstract

Conditions for assaying chemotaxis in B. subtilis are described. The chemotaxis medium that was used afforded excellent motility for hours. In it, chemotaxis measured by capillary assays was insensitive to pH between 5.5 and 9, and to temperature between 28°C and 42°C. Chemotaxis was observed toward all 20 common amino acids, with thresholds varying from 3 nM for alanine to 0.1 mM for glutamate, in the capillary assay, and from 0.1 μM for alanine to 0.32 mM for glutamate in the microscope assay.

Original languageEnglish (US)
Pages (from-to)151-155
Number of pages5
JournalJournal of bacteriology
Volume129
Issue number1
DOIs
StatePublished - 1977

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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