Chemical modification of essential arginine residues associated with the red beet (Beta vulgaris L.) plasma membrane Ca2+-ATPase

Swati Basu, Donald P. Briskin

Research output: Contribution to journalArticle

Abstract

Two α-dicarbonyl reagents, phenylglyoxal and 2,3-butanedione, were used to demonstrate the presence of essential arginine residues in the mechanism of the plasma membrane Ca2+-ATPase of red beet (Beta vulgaris L.) storage tissue. Both the ATP-dependent 45Ca2+ transport and ITP hydrolytic activities of the Ca2+-ATPase were inhibited by these reagents. Optimal inhibition was observed at pH 7.5 and 25°. Inhibition of ATP dependent 45Ca2+ transport by phenylglyoxal and 2,3-butanedione was decreased by inclusion of ATP in the incubation medium. These results demonstrate that arginine residues are involved in the mechanism of the red beet plasma membrane Ca2+-ATPase and may reside at the ATP binding region of the enzyme active site.

Original languageEnglish (US)
Pages (from-to)685-689
Number of pages5
JournalPhytochemistry
Volume41
Issue number3
DOIs
StatePublished - Jan 1 1996

Keywords

  • ATPase reaction mechanism, signal transduction
  • Beta Vulgaris
  • Chenopodiaceae
  • Red beet
  • Transport ATPase

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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