TY - JOUR
T1 - Chasing the elusive Euryarchaeota class WSA2
T2 - Genomes reveal a uniquely fastidious methyl-reducing methanogen
AU - Nobu, Masaru Konishi
AU - Narihiro, Takashi
AU - Kuroda, Kyohei
AU - Mei, Ran
AU - Liu, Wen Tso
N1 - Publisher Copyright:
© 2016 International Society for Microbial Ecology All rights reserved.
Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2016/10/1
Y1 - 2016/10/1
N2 - The ecophysiology of one candidate methanogen class WSA2 (or Arc I) remains largely uncharacterized, despite the long history of research on Euryarchaeota methanogenesis. To expand our understanding of methanogen diversity and evolution, we metagenomically recover eight draft genomes for four WSA2 populations. Taxonomic analyses indicate that WSA2 is a distinct class from other Euryarchaeota. None of genomes harbor pathways for CO 2 -reducing and aceticlastic methanogenesis, but all possess H 2 and CO oxidation and energy conservation through H 2 -oxidizing electron confurcation and internal H 2 cycling. As the only discernible methanogenic outlet, they consistently encode a methylated thiol coenzyme M methyltransferase. Although incomplete, all draft genomes point to the proposition that WSA2 is the first discovered methanogen restricted to methanogenesis through methylated thiol reduction. In addition, the genomes lack pathways for carbon fixation, nitrogen fixation and biosynthesis of many amino acids. Acetate, malonate and propionate may serve as carbon sources. Using methylated thiol reduction, WSA2 may not only bridge the carbon and sulfur cycles in eutrophic methanogenic environments, but also potentially compete with CO 2 -reducing methanogens and even sulfate reducers. These findings reveal a remarkably unique methanogen 'Candidatus Methanofastidiosum methylthiophilus' as the first insight into the sixth class of methanogens 'Candidatus Methanofastidiosa'.
AB - The ecophysiology of one candidate methanogen class WSA2 (or Arc I) remains largely uncharacterized, despite the long history of research on Euryarchaeota methanogenesis. To expand our understanding of methanogen diversity and evolution, we metagenomically recover eight draft genomes for four WSA2 populations. Taxonomic analyses indicate that WSA2 is a distinct class from other Euryarchaeota. None of genomes harbor pathways for CO 2 -reducing and aceticlastic methanogenesis, but all possess H 2 and CO oxidation and energy conservation through H 2 -oxidizing electron confurcation and internal H 2 cycling. As the only discernible methanogenic outlet, they consistently encode a methylated thiol coenzyme M methyltransferase. Although incomplete, all draft genomes point to the proposition that WSA2 is the first discovered methanogen restricted to methanogenesis through methylated thiol reduction. In addition, the genomes lack pathways for carbon fixation, nitrogen fixation and biosynthesis of many amino acids. Acetate, malonate and propionate may serve as carbon sources. Using methylated thiol reduction, WSA2 may not only bridge the carbon and sulfur cycles in eutrophic methanogenic environments, but also potentially compete with CO 2 -reducing methanogens and even sulfate reducers. These findings reveal a remarkably unique methanogen 'Candidatus Methanofastidiosum methylthiophilus' as the first insight into the sixth class of methanogens 'Candidatus Methanofastidiosa'.
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U2 - 10.1038/ismej.2016.33
DO - 10.1038/ismej.2016.33
M3 - Article
C2 - 26943620
AN - SCOPUS:84959529863
VL - 10
SP - 2478
EP - 2487
JO - ISME Journal
JF - ISME Journal
SN - 1751-7362
IS - 10
ER -