Characterizing stretched polynucleotides in a synthetic nanopassage

Gregory Timp (Inventor), Winston Timp (Inventor), Utkur M Mirsaidov (Inventor), Aleksei Aksimentiev (Inventor), Jeffrey R Comer (Inventor)

Research output: Patent

Abstract

Methods of trapping a deformed portion of a double-stranded polynucleotide in a membrane nanopassage are provided. In an aspect, the membrane has a nanopassage that defines a confine region, wherein the membrane separates a first fluid compartment from a second fluid compartment, and the nanopassage is in fluid communication with the first and second compartments. A polynucleotide is provided to the first fluid compartment and optionally a threshold voltage for the membrane and the polynucleotide is determined. A driving voltage across the membrane that is greater than the threshold voltage is applied to force a portion of the polynucleotide sequence into the nanopassage confine region, and decreased to a holding voltage bias to trap the polynucleotide portion in the nanopassage confine region. In particular, at least one nucleotide base-pair is fixably positioned in the nanopassage confine volume. In further embodiments, any of the trapping methods are used to characterize or sequence double stranded DNA.
Original languageEnglish (US)
U.S. patent number8748091
StatePublished - Jun 10 2014

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