@article{23bfa75178bd4b93bd1cf20c7ef7cb24,
title = "Characterizing Glioblastoma Heterogeneity via Single-Cell Receptor Quantification",
abstract = "Dysregulation of tyrosine kinase receptor (RTK) signaling pathways play important roles in glioblastoma (GBM). However, therapies targeting these signaling pathways have not been successful, partially because of drug resistance. Increasing evidence suggests that tumor heterogeneity, more specifically, GBM-associated stem and endothelial cell heterogeneity, may contribute to drug resistance. In this perspective article, we introduce a high-throughput, quantitative approach to profile plasma membrane RTKs on single cells. First, we review the roles of RTKs in cancer. Then, we discuss the sources of cell heterogeneity in GBM, providing context to the key cells directing resistance to drugs. Finally, we present our provisionally patented qFlow cytometry approach, and report results of a “proof of concept” patient-derived xenograft GBM study.",
keywords = "EGFR, IGFR, RTK, VEGFR, glioblastoma, heterogeneity, single-cell, stem cell",
author = "Si Chen and Thien Le and Harley, {Brendan A.C.} and Imoukhuede, {P. I.}",
note = "Funding Information: This work was supported by grants from the National Science Foundation (1512598 and 1653925), and the American Heart Association (16SDG26940002). Research reported in this publication was also supported by the National Cancer Institute of the National Institutes of Health under Award Number R01 CA197488 (BACH). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. We would like to thank members of BH's lab, Dr. Sara Pedron and Jee-Wei Emily Chen at University of Illinois at Urbana-Champaign, and Dr. Jann Sarkaria at Mayo Clinic (Rochester, MN) for the generous supply of GBM PDX samples. Funding Information: This work was supported by grants from the National Science Foundation (1512598 and 1653925), and the American Heart Association (16SDG26940002). Research reported in this publication was also supported by the National Cancer Institute of the National Institutes of Health under Award Number R01 CA197488 (BACH). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. We would like to thank members of BH{\textquoteright}s lab, Dr. Sara Pedron and Jee-Wei Emily Chen at University of Illinois at Urbana-Champaign, and Dr. Jann Sarkaria at Mayo Clinic (Rochester, MN) for the generous supply of GBM PDX samples. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2018 Chen, Le, Harley and Imoukhuede.",
year = "2018",
month = jul,
day = "11",
doi = "10.3389/fbioe.2018.00092",
language = "English (US)",
volume = "6",
journal = "Frontiers in Bioengineering and Biotechnology",
issn = "2296-4185",
publisher = "Frontiers Media S. A.",
}