Abstract
Engineered tissues can provide models for imaging and disease progression and the use of such models is becoming increasingly prevalent. While structural characterization of these systems is documented, a combination of biochemical and structural knowledge is often helpful. Here, we apply Fourier transform infrared (FT-IR) spectroscopic imaging to examine an engineered tissue model of melanoma. We first characterize the biochemical properties and spectral changes in different layers of growing skin. Second, we introduce malignant melanocytes to simulate tumor formation and growth. Both cellular changes associated with tumor formation and growth can be observed. In particular, chemical changes associated with tumor-stromal interactions are observed during the course of tumor growth and appear to influence a 50-100 μm region. The development of this analytical approach combining engineered tissue with spectroscopy, imaging and computation will allow for quality control and standardization in tissue engineering and novel scientific insight in cancer progression.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1569-1578 |
| Number of pages | 10 |
| Journal | Analyst |
| Volume | 135 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 1 2010 |
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ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry
Cite this
Characterization of tumor progression in engineered tissue using infrared spectroscopic imaging. / Kong, Rong; Reddy, Rohith K.; Bhargava, Rohit.
In: Analyst, Vol. 135, No. 7, 01.07.2010, p. 1569-1578.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Characterization of tumor progression in engineered tissue using infrared spectroscopic imaging
AU - Kong, Rong
AU - Reddy, Rohith K.
AU - Bhargava, Rohit
PY - 2010/7/1
Y1 - 2010/7/1
N2 - Engineered tissues can provide models for imaging and disease progression and the use of such models is becoming increasingly prevalent. While structural characterization of these systems is documented, a combination of biochemical and structural knowledge is often helpful. Here, we apply Fourier transform infrared (FT-IR) spectroscopic imaging to examine an engineered tissue model of melanoma. We first characterize the biochemical properties and spectral changes in different layers of growing skin. Second, we introduce malignant melanocytes to simulate tumor formation and growth. Both cellular changes associated with tumor formation and growth can be observed. In particular, chemical changes associated with tumor-stromal interactions are observed during the course of tumor growth and appear to influence a 50-100 μm region. The development of this analytical approach combining engineered tissue with spectroscopy, imaging and computation will allow for quality control and standardization in tissue engineering and novel scientific insight in cancer progression.
AB - Engineered tissues can provide models for imaging and disease progression and the use of such models is becoming increasingly prevalent. While structural characterization of these systems is documented, a combination of biochemical and structural knowledge is often helpful. Here, we apply Fourier transform infrared (FT-IR) spectroscopic imaging to examine an engineered tissue model of melanoma. We first characterize the biochemical properties and spectral changes in different layers of growing skin. Second, we introduce malignant melanocytes to simulate tumor formation and growth. Both cellular changes associated with tumor formation and growth can be observed. In particular, chemical changes associated with tumor-stromal interactions are observed during the course of tumor growth and appear to influence a 50-100 μm region. The development of this analytical approach combining engineered tissue with spectroscopy, imaging and computation will allow for quality control and standardization in tissue engineering and novel scientific insight in cancer progression.
UR - http://www.scopus.com/inward/record.url?scp=77953901778&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77953901778&partnerID=8YFLogxK
U2 - 10.1039/c0an00112k
DO - 10.1039/c0an00112k
M3 - Article
C2 - 20498913
AN - SCOPUS:77953901778
VL - 135
SP - 1569
EP - 1578
JO - Analyst
JF - Analyst
SN - 0003-2654
IS - 7
ER -