Characterization of the solubilized plasma membrane ATPase of red beet

Donald P. Briskin, Ronald J. Poole

Research output: Contribution to journalArticle

Abstract

The p1mm membrane ATP-phosphohydrolm (ATPae) frm red beet (Bta swirb L) storge tissue wasol d ith the zwitterionic eter t Zittergea 3-14 from a plas membne-enriched fion which e with the aok det t, sodium deoxycholate. For both the extraction of ext s pe by deoxycholate awl the soublo t of active plasm membrane ATPase by Zw tteg 3-14, the op l co rtin ofd nt was 0.1% (weigt per vome) with a dett to protein ratio of 1.0(per). The of the solublUzed ATPase were fonnd to be similar to the mebrane-bomd enyme wit respect to pH oium, subtrate specificity, ibitor sensitivity, an kietics of K+ stimulation. The solubiHzed ATPase pretio formed a rapidly uring over phospbheyme, the bradW veloiy of whic wa as in the presene of 50 millimolr KC. So2b Mtonwith 0.1% Zwtergent 3-14 fohowig extation with 0.1% deoxycolate resulted in an inem in both ATPae activity and steady sate pbospboenzyne level; however, a direct correspnee betwee the increas in ATPase tivity and pLhospborylatio lvel did nt exist. It Is that this diepacy may be the result of a deternt-m ated m ctIon of kinetic rate consats in the mechanism of the enzyme.

Original languageEnglish (US)
Pages (from-to)26-30
Number of pages5
JournalPlant physiology
Volume76
Issue number1
DOIs
StatePublished - Jan 1 1984

Fingerprint

Beta vulgaris
beets
adenosinetriphosphatase
Adenosine Triphosphatases
plasma membrane
Cell Membrane
deoxycholic acid
Deoxycholic Acid
Wit and Humor
Membranes
Proteins
proteins
Adenosine Triphosphate
kinetics
Sensitivity and Specificity
Enzymes
enzymes

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Characterization of the solubilized plasma membrane ATPase of red beet. / Briskin, Donald P.; Poole, Ronald J.

In: Plant physiology, Vol. 76, No. 1, 01.01.1984, p. 26-30.

Research output: Contribution to journalArticle

Briskin, Donald P. ; Poole, Ronald J. / Characterization of the solubilized plasma membrane ATPase of red beet. In: Plant physiology. 1984 ; Vol. 76, No. 1. pp. 26-30.
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