Abstract
The p1mm membrane ATP-phosphohydrolm (ATPae) frm red beet (Bta swirb L) storge tissue wasol d ith the zwitterionic eter t Zittergea 3-14 from a plas membne-enriched fion which e with the aok det t, sodium deoxycholate. For both the extraction of ext s pe by deoxycholate awl the soublo t of active plasm membrane ATPase by Zw tteg 3-14, the op l co rtin ofd nt was 0.1% (weigt per vome) with a dett to protein ratio of 1.0(per). The of the solublUzed ATPase were fonnd to be similar to the mebrane-bomd enyme wit respect to pH oium, subtrate specificity, ibitor sensitivity, an kietics of K+ stimulation. The solubiHzed ATPase pretio formed a rapidly uring over phospbheyme, the bradW veloiy of whic wa as in the presene of 50 millimolr KC. So2b Mtonwith 0.1% Zwtergent 3-14 fohowig extation with 0.1% deoxycolate resulted in an inem in both ATPae activity and steady sate pbospboenzyne level; however, a direct correspnee betwee the increas in ATPase tivity and pLhospborylatio lvel did nt exist. It Is that this diepacy may be the result of a deternt-m ated m ctIon of kinetic rate consats in the mechanism of the enzyme.
Original language | English (US) |
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Pages (from-to) | 26-30 |
Number of pages | 5 |
Journal | Plant physiology |
Volume | 76 |
Issue number | 1 |
DOIs | |
State | Published - 1984 |
Externally published | Yes |
ASJC Scopus subject areas
- Physiology
- Genetics
- Plant Science