Characterization of the pH-Dependent Resonance Raman Transitions of Archaeal and Bacterial Rieske [2Fe-2S] Proteins

Toshio Iwasaki, Asako Kounosu, Derrick R J Kolling, Antony R. Crofts, Sergei Dikanov, Akihisa Jin, Takeo Imai, Akio Urushiyama

Research output: Contribution to journalArticle

Abstract

The pH-dependent resonance Raman (RR) spectral changes of the cytochrome bc1-associated, high-potential Rieske proteins have frequently been invoked to explain the redox-linked ionization behavior. We report herein RR spectral data of archaeal and bacterial Rieske proteins that directly demonstrate the pH-dependent changes near and above pKa,ox2, but not around pKa,ox1, of the visible circular dichroism (CD) transitions. The RR spectral changes are attributed to modification of the immediate [2Fe-2S] cluster environment due to deprotonation of some exchangeable amide groups in the polypeptide backbone, rather than previously assumed simple changes of the Fe-Nimid stretching vibrations.

Original languageEnglish (US)
Pages (from-to)4788-4789
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number15
DOIs
StatePublished - Apr 21 2004

ASJC Scopus subject areas

  • Chemistry(all)

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