Characterization of the GH16 and GH17 laminarinases from Vibrio breoganii 1C10

Ahmet H. Badur, Ehab M. Ammar, Geethika Yalamanchili, Jan Hendrik Hehemann, Christopher V. Rao

Research output: Contribution to journalArticle

Abstract

Laminarin is an abundant glucose polymer used as an energy reserve by micro- and macroalgae. Bacteria digest and consume laminarin with laminarinases. Their genomes frequently contain multiple homologs; however, the biological role for this replication remains unclear. We investigated the four laminarinases of glycoside hydrolase families GH16 and GH17 from the marine bacterium Vibrio breoganii 1C10, which can use laminarin as its sole carbon source. All four laminarinases employ an endolytic mechanism and specifically cleave the β-1,3-glycosidic bond. Two primarily produce low–molecular weight laminarin oligomers (DP 3–4) whereas the others primarily produce high–molecular weight oligomers (DP > 8), which suggests that these enzymes sequentially degrade laminarin. The results from this work provide an overview of the laminarinases from a single marine bacterium and also provide insights regarding how multiple laminarinases are used to degrade laminarin.

Original languageEnglish (US)
Pages (from-to)161-171
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume104
Issue number1
DOIs
StatePublished - Jan 1 2020

Fingerprint

Cellulases
Vibrio
Bacteria
Microalgae
Weights and Measures
Seaweed
Glucans
Glycoside Hydrolases
laminaran
Carbon
Genome
Enzymes

Keywords

  • Enzyme
  • Glycoside hydrolase
  • Laminarin
  • Laminarinase

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Characterization of the GH16 and GH17 laminarinases from Vibrio breoganii 1C10. / Badur, Ahmet H.; Ammar, Ehab M.; Yalamanchili, Geethika; Hehemann, Jan Hendrik; Rao, Christopher V.

In: Applied Microbiology and Biotechnology, Vol. 104, No. 1, 01.01.2020, p. 161-171.

Research output: Contribution to journalArticle

Badur, Ahmet H. ; Ammar, Ehab M. ; Yalamanchili, Geethika ; Hehemann, Jan Hendrik ; Rao, Christopher V. / Characterization of the GH16 and GH17 laminarinases from Vibrio breoganii 1C10. In: Applied Microbiology and Biotechnology. 2020 ; Vol. 104, No. 1. pp. 161-171.
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