Characterization of stable high molecular weight mutagenic product(s) of plant-activated m-phenylenediamine

Kwang Young Seo, Joan Riley, David Cortez, Elizabeth D. Wagner, Michael J. Plewa

Research output: Contribution to journalArticle

Abstract

Monocyclic aromatic amines are environmental contaminants and many are promutagens and procarcinogens. Cultured tobacco cells, strain TX1, activated m-phenylenediamine into a frameshift mutagen that reverted the hisD3052 allele in Salmonella typhimurium strains TA98 and YG1024. However, the plant-activated products were refractory in strain TA98/1,8-DNP6. This indicated that these plant-activated products were substrates for bacterial acetyl-CoA: N-hydroxyarylamine O-acetyltransferase. A stable, high molecular weight ( > 300 kDa) proximal mutagen was isolated by molecular ultrafiltration membranes. No parent compound was associated with the isolated mutagenic fraction. The high molecular weight fraction induced mutation in S. typhimurium strains TA98, YG1021 and YG1024. From these data we propose a model for the plant-activation of aromatic amine promutagens.

Original languageEnglish (US)
Pages (from-to)111-120
Number of pages10
JournalMutation Research/Genetic Toxicology
Volume299
Issue number2
DOIs
StatePublished - Apr 1993

Keywords

  • Frameshift mutations
  • Nicotiana tabacum
  • Plant cell/microbe coincubation assay
  • Promutagens

ASJC Scopus subject areas

  • Toxicology
  • Genetics

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