Characterization of quinone outside inhibitor fungicide resistance in Cercospora sojina and development of diagnostic tools for its identification

F. Zeng, E. Arnao, G. Zhang, G. Olaya, J. Wullschleger, H. Sierotzki, Ray R Ming, B. H. Bluhm, J. P. Bond, A. M. Fakhoury, Carl Bradley

Research output: Contribution to journalArticle

Abstract

Frogeye leaf spot of soybean, caused by the fungus Cercospora sojina, reduces soybean yields in most of the top-producing countries around the world. Control strategies for frogeye leaf spot can rely heavily on quinone outside inhibitor (QoI) fungicides. In 2010, QoI fungicide-resistant C. sojina isolates were identified in Tennessee for the first time. As the target of QoI fungicides, the cytochrome b gene present in fungal mitochondria has played a key role in the development of resistance to this fungicide class. The cytochrome b genes from three QoI-sensitive and three QoI-resistant C. sojina isolates were cloned and sequenced. The complete coding sequence of the cytochrome b gene was identified and found to encode 396 amino acids. The QoI-resistant C. sojina isolates contained the G143A mutation in the cytochrome b gene, a guanidine to cytosine transversion at the second position in codon 143 that causes an amino acid substitution of alanine for glycine. C. sojina-specific polymerase chain reaction primer sets and TaqMan probes were developed to efficiently discriminate QoI-resistant and -sensitive isolates. The molecular basis of QoI fungicide resistance in field isolates of C. sojina was identified as the G143A mutation, and specific molecular approaches were developed to discriminate and to track QoI-resistant and -sensitive isolates of C. sojina.

Original languageEnglish (US)
Pages (from-to)544-550
Number of pages7
JournalPlant disease
Volume99
Issue number4
DOIs
StatePublished - Jan 1 2015

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Cercospora sojina
fungicide resistance
quinones
cytochrome b
frogeye leaf spot
fungicides
genes
soybeans
mutation
guanidines
cytosine
amino acid substitution
glycine (amino acid)
codons
alanine
mitochondria
polymerase chain reaction

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

Cite this

Characterization of quinone outside inhibitor fungicide resistance in Cercospora sojina and development of diagnostic tools for its identification. / Zeng, F.; Arnao, E.; Zhang, G.; Olaya, G.; Wullschleger, J.; Sierotzki, H.; Ming, Ray R; Bluhm, B. H.; Bond, J. P.; Fakhoury, A. M.; Bradley, Carl.

In: Plant disease, Vol. 99, No. 4, 01.01.2015, p. 544-550.

Research output: Contribution to journalArticle

Zeng, F, Arnao, E, Zhang, G, Olaya, G, Wullschleger, J, Sierotzki, H, Ming, RR, Bluhm, BH, Bond, JP, Fakhoury, AM & Bradley, C 2015, 'Characterization of quinone outside inhibitor fungicide resistance in Cercospora sojina and development of diagnostic tools for its identification', Plant disease, vol. 99, no. 4, pp. 544-550. https://doi.org/10.1094/PDIS-05-14-0460-RE
Zeng, F. ; Arnao, E. ; Zhang, G. ; Olaya, G. ; Wullschleger, J. ; Sierotzki, H. ; Ming, Ray R ; Bluhm, B. H. ; Bond, J. P. ; Fakhoury, A. M. ; Bradley, Carl. / Characterization of quinone outside inhibitor fungicide resistance in Cercospora sojina and development of diagnostic tools for its identification. In: Plant disease. 2015 ; Vol. 99, No. 4. pp. 544-550.
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