Characterization of peptides from Aplysia using microbore liquid chromatography with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry guided purification

Philip D. Floyd, Lingjun Li, Tatiana P. Moroz, Jonathan V. Sweedler

Research output: Contribution to journalArticlepeer-review

Abstract

Liquid chromatography (LC) has been used extensively for the separation and isolation of peptides due to its high selectivity and peak capacity. An approach combining microbore LC with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-MS) detection is described to identify peptides in cells and guide the purification of peptides from the marine mollusc Aplysia californica. Direct MALDI-MS of neurons and processes provides molecular mass information for unknown peptides with almost no sample preparation, and LC-MALDI-MS allows the isolation and purification of these peptides from pooled samples, thus enabling new putative neuropeptides to be isolated from complex cellular samples. Both direct MALDI-MS and LC-MALDI-MS are compared in terms of detecting peptides from neuronal samples. Using both approaches, two peaks from Aplysia californica connectives having molecular masses of 5013 and 5021 have been isolated, partially sequenced and identified as novel collagen-like peptides. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)105-113
Number of pages9
JournalJournal of Chromatography A
Volume830
Issue number1
DOIs
StatePublished - Jan 8 1999

Keywords

  • Aplysia californica
  • Collagen
  • Liquid chromatography-mass spectrometry
  • Matrix-assisted laser desorption/ionization mass spectrometry
  • Neuropeptides
  • Peptides

ASJC Scopus subject areas

  • Analytical Chemistry

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