TY - JOUR
T1 - Characterization of monoclonal antibodies directed against pyruvate oxidase from Escherichia coli
T2 - Modulation of antibody-induced inhibition by enzyme conformation
AU - Barassi, Carlos A.
AU - Kranz, Robert G.
AU - Gennis, Robert B.
N1 - Funding Information:
We would like to thank Patrick Porter for preparing the pyruvate oxidase used in this work. This work was supported by grants Prom the National Institutes of Heath, HL16101 and the American Heart Association. C.A.B. was supported by a fellowship award from the Consejo National de Investigaciones Cientificas y Tecnicas de la Republica Argentina.
PY - 1986/6/13
Y1 - 1986/6/13
N2 - Monoclonal antibodies have been prepared against pyruvate oxidase, a flavoprotein dehydrogenase isolated from Escherchia coli. Six monoclonals were obtained, but only one was found to bind to the native form of the enzyme. This monoclonal, 1I1, was a potent inhibitor. Although this antibody inhibited the unactivated and lipid-activated forms of the enzyme, it had much less of an inhibitory effect on the protease-activated form of the enzyme, although the antibody still bound to this form. Hence, the coupling between antibody binding and the conformation at the active site can itself be modulated by the conformation of the protein.
AB - Monoclonal antibodies have been prepared against pyruvate oxidase, a flavoprotein dehydrogenase isolated from Escherchia coli. Six monoclonals were obtained, but only one was found to bind to the native form of the enzyme. This monoclonal, 1I1, was a potent inhibitor. Although this antibody inhibited the unactivated and lipid-activated forms of the enzyme, it had much less of an inhibitory effect on the protease-activated form of the enzyme, although the antibody still bound to this form. Hence, the coupling between antibody binding and the conformation at the active site can itself be modulated by the conformation of the protein.
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U2 - 10.1016/0006-291X(86)91162-9
DO - 10.1016/0006-291X(86)91162-9
M3 - Article
C2 - 3524564
AN - SCOPUS:0022448881
SN - 0006-291X
VL - 137
SP - 884
EP - 891
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -