Characterization of maize cytochrome P450 monooxygenases induced in response to safeners and bacterial pathogens

M. W. Persans, J. Wang, M. A. Schuler

Research output: Contribution to journalArticle

Abstract

Plants use a diverse array of cytochrome P450 monooxygenases in their biosynthetic and detoxification pathways. To determine the extent to which various maize P450s are induced in response to chemical inducers, such as naphthalic anhydride (NA), triasulfuron (T), phenobarbital, and bacterial pathogens (Erwinia stuartii, Acidovorax avenae), we have analyzed the response patterns of seven P450 transcripts after treatment of seedlings with these inducers. Each of these P450 transcripts has distinct developmental, tissue-specific, and chemical cues regulating their expression even when they encode P450s within the same biosynthetic pathway. Most notably, the CYP71C1 and CYP71C3 transcripts, encoding P450s in the DIMBOA biosynthetic pathway, are induced to the same level in response to wounding and NA treatment of younger seedlings and differentially in response to NA/T treatment of younger seedlings and NA and NA/T treatment of older seedlings. NA and T induce expression of both CYP92A1 and CYP72A5 transcripts in older seedling shoots, whereas phenobarbital induces CYP92A1 expression in older seedling shoots and highly induces CYP72A5 expression in young and older seedling roots. Expressed sequence tag (EST) 6c06b11 transcripts, encoding an undefined P450 activity, are highly induced in seedling shoots infected with bacterial pathogens.

Original languageEnglish (US)
Pages (from-to)1126-1138
Number of pages13
JournalPlant physiology
Volume125
Issue number2
DOIs
StatePublished - Mar 6 2001

Fingerprint

naphthalic anhydride
Mixed Function Oxygenases
Seedlings
cytochrome P-450
Cytochrome P-450 Enzyme System
Zea mays
corn
seedlings
pathogens
Biosynthetic Pathways
phenobarbital
Phenobarbital
shoots
biochemical pathways
Acidovorax avenae
Comamonadaceae
triasulfuron
Erwinia
Expressed Sequence Tags
expressed sequence tags

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Cite this

Characterization of maize cytochrome P450 monooxygenases induced in response to safeners and bacterial pathogens. / Persans, M. W.; Wang, J.; Schuler, M. A.

In: Plant physiology, Vol. 125, No. 2, 06.03.2001, p. 1126-1138.

Research output: Contribution to journalArticle

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